PECULIARITIES OF MARKER EXPRESSION OF CD34 ENDOTHELIAL CELLS IN THE VASCULAR LEVEL OF THE VILLOSE TREE PLACENTA DURING PREGNANCY INDUCED IN THE CYCLE OF AUXILIARY REPRODUCTIVE TECHNOLOGIES
DOI:
https://doi.org/10.11603/24116-4944.2020.2.11857Keywords:
infertility, assisted reproductive technologies, pregnancy, placenta, chorionic villus, vascularization, endothelium, CD34Abstract
The aim of the study – to learn the features of CD34 endothelial cell marker expression in the vascular bed of the placental villous tree in full-term pregnancy after ART.
Materials and Methods. The material was 65 placentas of the ART group from singleton full-term pregnancies induced in cycles of fertilization in vitro, and 30 placentas from singleton full-term pregnancies after natural conception in relatively healthy women. The placentas of the ART group were divided into 2 subgroups: from women in labor with cured primary infertility (n = 33) and from cured secondary infertility (n = 32). Immunohistochemical methods were used to assess the expression area and optical density of CD34 in the villous chorion of the placenta.
Research and Discussion. The largest area of CD34 expression in the villous chorion of the placenta was observed in the control group and amounted to (9.49±0.47) %, in the placentas of women with cured primary infertility, it was lower by 1.11 times (8.51±0.17) %, p<0.01) and with cured secondary infertility – 1.29 times (7.34±0.15) %, p<0.01). In turn, the area of CD34 expression in the villous chorion of the placenta from women with cured primary infertility exceeded that from women with secondary infertility 1.16 times (p<0.01). The highest optical density of CD 34 expression in the villous chorion of the placenta was observed in women with cured primary infertility – (0.22±0.01) conventional units, which exceeded that in patients with secondary infertility (0.19±0.01) conventional units) 1.33 times (p<0.01) and in the control (0.20±0.01) conventional units) – 1.25 times (p<0.01). The obtained data are similar to the results of placental studies in women with pregnancy after ART and early preterm birth, where there was a high frequency of dysplastic processes with alteration, dissociated maturation of villous villi, sclerosis of the villous stroma, dyscirculatory disorders and significant reduction. Our own and published data indicate that in the placentas of women with pregnancy induced in ART cycles, there are violations of the vascularization of the chorionic villus with changes in the expression of CD34 of varying severity, which are observed both in premature and full-term, both during the first and during the second pregnancy. This requires the implementation of appropriate treatment and prevention measures during gestation.
Conclusions. Features of CD34 production in the cohort of women with primary infertility and full-term pregnancy cured in ART cycles are a decrease in its expression area with a simultaneous increase in the intensity of immunostaining, and in a cohort of women with cured secondary infertility – a decrease in expression area with intensity not different from physiology.
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