The determination of content of anthocyanins and tannins In fruit of aronia melanocarpa
DOI:
https://doi.org/10.11603/mcch.2410-681X.2018.v0.i1.8756Keywords:
Aronia melanocarpa, anthocyanins, tannins, polyphenols, standardization.Abstract
Introduction. Aronia melanocarpa is widely cultivated in Ukraine as a food, medicinal and ornamental plant. The quality of fresh fruit of Aronia melanocarpa was regulated by the requirements of the pharmaceutical article 42-66-87 "Fresh fruit of Aronia melanocarpa", but the article required a revision using modern approaches to the standardization of medicinal plant material.
The aim of the study – determination of the content of anthocyanins and tannins in fresh and dried fruit of Aronia melanocarpa for inclusion the results of the research in the section of the monograph "Quantitative determination".
Research Methods. The determination of the content of anthocyanins and tannins in fresh and dried fruit of Aronia melanocarpa was carried out by the method of absorption spectrophotometry.
Results and Discussion. The content of anthocyanins in fresh fruit of Aronia melanocarpa is from (0.45±0.01) % to (0.56±0.02) % in terms of cyanidin-3-O-glucoside chloride, the content of tannins in dried fruit of Aronia melanocarpa is from (1.51±0.02) % to (2.41±0.03) % in terms of pyrogallol and dry raw materials. Therefore we proposed to enter the indicator: the content of anthocyanins – at least 0.40 % in terms of cyanidin-3-O-glucoside chloride to the national monograph "Fresh fruit of Aronia melanocarpaN", and the indicator: the content of tannins – not less than 1.5 % in terms of pyrogallol and dry raw materials to the national monograph "Dried fruit of Aronia melanocarpaN".
Conclusion. By the method of absorption spectrophotometry in fresh fruit of Aronia melanocarpa the content of anthocyanins was determined, in dried fruit of Aronia melanocarpa – tannins. The results of the research were used during the development of the national monographs "Fresh fruit of Aronia melanocarpaN" and "Dried fruit of Aronia melanocarpaN".
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