Peroxide oxidation of proteins in the small intestine wall, heart and liver in experimental intake of carrageenan
DOI:
https://doi.org/10.11603/mcch.2410-681X.2017.v0.i4.8435Keywords:
oxidation modification of proteins, carrageenan, experiment.Abstract
Introduction. In the last decades, the carrageenans have become one of the most popular hydrocolloids in the food industry. In addition to the positive properties of hydrocolloids, researchers have established the relationship between the incidence of ulcerative colitis and the level of carrageenan consumption, which makes it considered as a potential etiological factor in the pathology of the gastrointestinal tract.
The aim of the study – to determine the level of oxidative modification of proteins of the wall of the small intestine, tissues of the heart and liver of rats using 1% κ-carrageenan solution.
Materials and Methods. The study was carried out on 24 white non-linear male rats. The animals of the experimental group were provided with free access to a 1.0% of carrageenan solution for 1 month. In the selected samples of the small intestine, heart and liver was evaluated the peroxidic oxidation of proteins according to the level of their oxidation modifications products using spectrophotometry at wavelengths of 370 and 430 nm.
Research Results. A significant increasing of ketone dinitrophenylhydrazones levels in the small intestine wall (by 57.0%) and in the liver (by 23.0%) and, respectively, the aldehyde dinitrophenylhydrazones levels by 47.7% and 19.1%, compared with the control values (p<0.01). In this case, the peroxide oxidation, to a greater extent, was subjected to neutral proteins. It should be noted that the peroxidic oxidation of proteins in the myocardium of the rats with carrageenan consumption, recorded at a wavelength of 370 nm, exceeded the control data by 14.3% (p<0.05).
Conclusions. Oral administration of a 1% carrageenan solution in experimental animals leads to statistically significant activation statistically significant activation of peroxidation of proteins in the wall of the small intestine and the liver, whereas only the growth of neutral ketone dinitrophenylhydrazones is registered in the myocardium (p<0.05).
References
Fillips, G.O., & Vilyams, P.A. (2006). Spravochnik po gidrokolloidam [Handbook of hydrocolloids]. SPb: GIORD [in Russian].
Dorohovich, A.M., Solovyova, O.L., Bondaruk, Yu.K. Zheleyniy marmelad [Jelly Marmalade]. Patent Ukrayina, № u201003479, 2010.
Bohomolova, V.V. Sposib pryhotuvannia rybnykh konserviv v zhele [Method of preparation of canned fish in jelly]. Patent Ukrayina, № a 200900303, 2009.
Pasalskyi, V.K. (2009). Khimiia kharchovykh produktiv [Chemistry of food products]. K: KDTEU [in Ukrainian].
Rogov, I.A., Zharinov, A.I., Tekuteva, L.A., & Shepel, T.A. (2009). Biotehnologiya myasa i myasoproduktov: kurs lektsIy [Biotechnology of meat and meat products: a course of lectures]. M.: DeLi print [in Russian].
Tekuteva, L.A. (2003). Vyisokoochischennyie karraginanyi – skryityiy rezerv ekonomicheskoy effektivnosti [Highly purified carrageenans - a hidden reserve of economic efficiency]. Myasnoy ryad – Meat row, 3, 36-37 [in Russian].
Kucheruk, Z.I., & Tsukanova, O.S. (2014). Vykorystannia polisakharydiv roslynnoho i mikrobnoho pokhodzhennia v tekhnolohii bezbilkovoho khliba: monohrafiia [The use of polysaccharides of plant and microbial origin in the technology of non-protein bread: a monograph]. Kharkiv: KhDUKhT.
Sabadosh, H.O. (2010). Tekhnolohiia desertiv molochnykh z vykorystanniam karahinaniv [Technology of dairy desserts using carrageenans]. Extended abstract of candidate’s thesis. Kharkiv [in Ukrainian].
Svetlakov, D.B. (2004). Razrabotka kompozitsiy na osnove kappa-karraginana dlya regulirovaniya reologicheskih svoystv emulgirovannyih myasoproduktov [Development of compositions based on kappa-carrageenan for regulating the rheological properties of emulsified meat products]. Extended abstract of candidate’s thesis. M. [in Russian].
Colloids, M. (2007). Karraginan. Tehnologicheskaya informatsiya [Carrageenan. Technological information]. Philadelphia: Retrieved fromhttp://biokhim.com/data2/fmc/FMC%20biopolymer.pdf
Denysenko, O.I. (2004). Okysna modyfikatsiia bilkiv yak chynnyk patohenezu alerhodermatoziv [Oxidative modification of proteins as a factor in the pathogenesis of allergic dermatitis]. Ukrainskyi zhurnal dermatolohii, venerolohii, kosmetolohii – Ukrainian Journal of Dermatology, Venereology, Cosmetology, 1, 23-26 [in Ukrainian].
Ryabov, G.Ya., Azimov, Yu.M., Dorohov, S.I, еt al. (2000). Okislitelnaya modifikatsiya belkov plazmyi krovi u bolnyih v kriticheskih sostoyaniyah [Oxidative modification of plasma proteins in patients in critical conditions] Anesteziologiya i reanimatologiya – Anesthesiology and Reanimatology, 2, 72-75 [in Ukrainian].
Moyana, T.N., & Lalonde, J.M. (1990). Carrageenan-induced intestinal injury in the rat – a model for inflammatory bowel disease. Ann Clin Lab Sci, 20, 6, 420-426.
Gubina-Vakuly`k, G.I., Kolousova, N.G., Ivanenko, Т.О., Gorbach, T.V., Korobchans`ky`j, V.O. Sposib modelyuvannya xronichnogo gastroenterokolitu [Method of modeling chronic gastroenterocolitis]. Patent Ukrayina, № a201014510, 2012.
Nesterova, L.A., Smurova, E.A., & Manukhin, B.N. (1995). Characterization of specific binding blocker [+ H]-quinuclidinylbenzilate Mcholinergic receptors in rat brain cortex membranes. Reports of the Academy of Sciences, 343 (2), 268-271 [In Russian].
Lowry, O.H. (1951). Protein measurement with the Folin phenol reagent. Journal of Biоlogical Chemistry, 193 (1), 404-415.
Cemeniuk, H.D., Melnychuk, H.M., & Ersteniuk, H.M. (2013). Stan intensyvnosti okysniuvalnoi modyfikatsii bilkiv ta aktyvnosti antyoksydantnykh fermentiv u rotovii ridyni khvorykh na heneralizovanyi parodontyt [State of intensity of oxidative modification of proteins and activity of antioxidant enzymes in the oral liquid of patients with generalized periodontitis]. Arkhiv klinichnoi medytsyny – Archive of clinical medicine, 69-71 [in Ukrainian].
Glanc, S. (1999). Mediko-biologicheskaja statistika [Medical and Biological Statistics]. Moskva: Praktika, 459 [in Russian].
Rebrova, O.Yu. (2002). Statisticheskiy analiz meditsinskih dannyh. Primenenie paketa prikladnyh programm STATISTICA [Statistical analysis of medical data. Application of software package STATISTICA]. M: MediaSfera [in Russian].
Mialiuk, O.P. (2015). Stan vilnoradykalnoho okysnennia u tkanynakh pechinky eksperymentalnykh shchuriv pry alimentarnomu ozhyrinni [Condition of free radical oxidation in liver tissues of experimental rats under alimentary obesity]. Visnyk problem biolohii i medytsyny – Bulletin of Biology and Medicine, 4 (1), 120-123 [in Ukrainian].
Marakushyn, D.I. (2015). Vplyv oksyetylvanykh nonilfenoliv ta yikh pokhidnykh na protsesy lipoperoksydatsii ta okysnoi modyfikatsii bilkiv v orhanizmi shchuriv [The influence of oxyethylvanofenylphenols and their derivatives on lipoperoxidation and oxidative modification of proteins in the rat body]. Zdobutky klinichnoi i eksperymentalnoi medytsyny – Achievements of clinical and experimental medicine, 1, 94-97 [in Ukrainian].
Zyn, A., Holovchak, D., & Sanahurskyi, D. (2013). Okysna modyfikatsiia bilkiv u zarodkakh v’iuna MISGURNUS FOSSILIS L. uprodovzh embriohenezu za dii hipokhlorytu natriiu [Oxidative modification of proteins in the embryos of MISGURNUS FOSSILIS L. During the embryogenesis of the action of sodium hypochlorite]. Visnyk Lvivskoho universytetu. Seriia biolohichna – Visnyk of Lviv University. Biological series, 61, 11-19 [in Ukrainian].
