ESTIMATION OF THE SPECIES COMPOSITION OF THE MICROFLORA OF THICK INTESTINE IN THE RATS AT THE MULTIPLE INHALATION REACTION OF NITROXOLINE
DOI:
https://doi.org/10.11603/1811-2471.2018.v0.i4.9602Keywords:
nitroxoline, inhalation effect, colon microbiocenosisAbstract
An important in the scheme of research on the hygienic justification of hygienic regulations for the admissible content of drugs in the air of the working zone of pharmaceutical enterprises is the establishment of parameters of toxicity, allergic, embryotoxic, dysbiotic action.
Aim. Evaluation of the microflora species composition of the direct colon in rats with repeated inhalation exposure to nitroxoline.
Materials and methods. The dysbiotic effect of nitroxolin was studied in rats, which were exposed to the drug at concentrations of 5 mg/m3, 15 mg/m3, 45 mg/m3 for 30 days by intranasal administration.
Results and discussion. The dominant representatives of the fecal biotope normobites are Bifidobacteria, Lactobacteria, bacteria of the intestinal sticks, in particular E. coli, Enterococcus, and Staphylococcus, which were detected in the excrements of 100.0% of animals. The frequency of occurrence of Peptostreptococcus and Streptococcus ranged from 33.3 % - 66.7 %, Protea - 16.7% - 50.0 %, Candida fungi - 16.7 % - 33.3 %, Mold – 0 % - 16,7 %. When exposed to nitroxoline concentrations of 5 mg/m3, 15 mg/m3, 45 mg/m3, the prevalence rate for the dominant bacterial populations: Bifidobacteria, Lactobacillus, bacteria in the E. coli group, Enterococcus and Staphylococcus remained unchanged at 100.0%. Nitroxolin at a concentration of 5 mg/m3 did not cause changes in the structure of the intestinal microbiota. Concentrations of 15 mg/m3, 45 mg/m3 caused an increase in the frequency of detection of opportunistic strains and reduction of Peptostreptococcus and Streptococcus. At the end of the recovery period, the frequency of occurrence of Proteus, Candida fungi, Mold fungi in the fecal biotope of rats of the second (15 mg/m3) and third experimental group (45 mg/m3) was kept at the level of 4 weeks of the experimental level.
Conclusions. Multiple inhalation action of nitroxolin in concentration 15 mg/m3 causes dysbiosis of the large intestine in rats, with a concentration of compound of 15 mg/m3 is a threshold for this effect.
