PRO12ALA PPAR-γ2 GENE POLYMORPHISM IN PATIENTS WITH NON-ALCOHOLIC LIVER DISEASE, ARTERIAL HYPERTHERMIA AND EXPOSURE
DOI:
https://doi.org/10.11603/1811-2471.2018.v0.i2.8933Keywords:
non-alcoholic fatty liver disease, gene PPAR-γ2 (Pro12Ala), arterial hypertension, obesity.Abstract
Today, there is a lot of controversial information on the role of PPAR-γ2 gene polymorphisms in the development of metabolic syndrome, dyslipidemia, arterial hypertension (hypertension), and obesity.
The aim – to analyze the frequency of alleles and genotypes of the Pro12Ala polymorphism of the PPAR-γ2 gene in the structure of patients with NAGHP with essential AG, burdened with abdominal obesity (AO).
Material and Methods. In 96 patients with NADH in combination with the essential AG II stage and AO 1-2 degrees performed the study of Pro12Ala polymorphism of the PPAR-γ2 gene by PCR. Men were 41.67 %, women – 58.33 %. The average age of patients was (53.70±5.34) years. The control group consisted of 50 practically healthy subjects, comparable in age (47.99±8.46 years) and sex (60 % women, 40 % men) who were not related to the patients.
Results. Among the examined steatohepatitis with the minimum activity of the mesenchymal-inflammatory process, 16.67 % (16) patients were registered, and in 83.33% (80) of cases steatohepatosis was detected. 27.08 % (26) persons had AO I degree, 58.33 % (56) people – AO II degree, 14.58 % (14) patients – AO III degree.
In general, both among the control group and among patients, the Pro-allele was dominated by 6.14 and 3.85 times (p <0.001), respectively, without a significant difference in the frequency of individual genotypes. However, in the control group, the wild Pro-allele, less often Ala-allele, was significantly more frequent than in the patients with NAGHP with essential AG, AO I degree in 16.77% (χ2 = 5.06, p = 0.024). The frequency of alleles and genotypes of the PPAR-γ2 gene (rs1801282) between patients with steatohepatitis and steatohepatose was not significantly different. However, the relative frequency of individuals with AlaAla- and ProAla-genotypes and, respectively, with the Ala-allele was significantly higher in patients with steatohepatitis than in the control – by 30.25% (χ2 = 4.99; p = 0.025) and 17,25% (χ2 = 4.85; p = 0.028). However, the relative frequency of persons with ProPro-genotype and Pro-genome per revolution was less than in control by 30.25% (p = 0.025) and 17.25% (p = 0.028).
Conclusions. The Ala-allele of the PPAR-γ2 gene (Pro12Ala) is associated with a higher incidence of steatohepatitis in patients with hypertension and AO.
References
Thorand, B., Zeirer, A., & Baumertetal, J. (2010). As¬sociations between leptin and leptin/adiponectin ratio and incident type 2 diabetes in middle-age men and women: results from the MONICA/KORA Augsburg study 1984–2002. Diabetic Medicine, 27, 1004-1011.
Vuppalanchi, R., & Chalasani, N. (2009). Non-alco¬holic fatty liver disease and nonalcoholic steatohepatitis: selected practical issues in their evaluation and manage¬ment. Hepatology, 49, 306-317.
de Alwis, N.M., & Day, C.P. (2008). Non-alcoholic fatty liver disease: the mist gradually clears. J. Hepatol., 48 (1), 104-112.
Petta, S., Muratore, C., & Craxi, A. (2009). Non-alco¬holic fatty liver disease pathogenesis: the present and the future. Dig. Liver Dis., 41, 615-625.
Dowman, J.K., Tomlinson, J.W., & Newsome, P.N. (2010). Pathogenesis of non-alcoholic fatty liver dis-ease. QJM, 103, 71-83.
Day, C.P. (2006). From fat to inflammation. Gastro¬enterology, 130, 207-210.
Browning, J.D., Szczepaniak, L.S., & Dobbins, R. (2004). Prevalence of hepatic steatosis in an urban popula¬tion in the United States: impact of ethnicity. Hepatology, 40, 1387-1395.
Bedogni, G., Miglioli, L., & Masutti, F. (2005). Preva¬lence of and risk factors for nonalcoholic fatty liver dis¬ease: the Dionysos nutrition and liver study. Hepatology, 42, 44-52.
Machado, M., & Marques-Vidal, P. (2006). Hepatic histology in obese patients undergoing bariatric surgery. J. Hepatol., 45, 600-606.
Musso, G., Gambino, R., & Cassader, M. (2011). Need for a three-focused approach to nonalcoholic fatty liver disease. Hepatology, 53 (5), 1773.
Adams, L.A., Lymp, J.F., & Sauver, J. St. (2005). The natural history of nonalcoholic fatty liver disease: a population-based cohort study. Gastroenterology, 129 (1), 113-121.
