BIOCHEMICAL CHANGES IN BLOOD SERUM OF RATS WITH REPERFUSION-ISCHEMIC SYNDROME (THE EXPERIMENTAL STUDY)
DOI:
https://doi.org/10.11603/1811-2471.2018.v0.i2.8775Keywords:
ischemic-reperfusion syndrome, muscle tissue, biochemical changes, metabolism.Abstract
A number of studies have shown that violation of cellular metabolism, accumulation in non-physiological concentrations of metabolic products, and development of endogenous intoxication in ischemic-reperfusion syndrome (IRS) are important mechanisms for tissue alteration.
The aim of the study – to determine the degree of metabolic disturbances based on the analysis of biochemical changes in the blood serum of the rats with IRS, caused by overlaying the arterial tourniquet on the hind limbs.
Material and Methods. Under conditions of the experimental ischemic-reperfusion, the biochemical examination of the blood serum of 30 experimental rats injury was conducted. In the control group there were 6 intact animals. The ischemia-reperfusion syndrome was modeled by applying of SWAT rubber tourniquet on the hind limbs of the animals. Blood collection accomplished by means of cardiotomy. Euthanasia of the animals was performed by decapitation under the thiopental-sodium anestesia. The biochemical changes, namely the indices of total bilirubin, triglycerides (TG), cholesterol, creatinine, total protein (TP), alkaline phosphatase (AF), and the level of transaminases (AlAT, AsAT) were determined in each group. The study of indicators was carried out in the inter-departmental scientific-clinical laboratory of I. Horbachevsky Ternopil State Medical University by reagents of Scientific-Production Enterprise "Filisit-Diagnostika" LLC of the city of Dnipro. The quantitative indicators were processed statistically.
Results and Discussion. Researches have shown an increasing activity of transaminases, AF and total bilirubin and decreasing concentration of creatinine and TP in the early reperfusion period. In particular, the level of AlAT reached its the highest value in the first day, which was 89.5 % higher than the control group (P<0.05), and the AsAT index was the highest in the animals with 2-hour reperfusion, which was 78.73 % higher corresponding indicator of the control group (Р<0.005). AF reached its maximum on the 7th day, which is 45.78 % higher than the index of the control group. Increase in concentration of total bilirubin was noted in the animals on the 7th day- the indicator exceeded the control by 105.3 % (Р<0.005)
Conclusions. Changes in biochemical indices were most pronounced in the animals of the early period model of postischemic lesions (2nd hour and 1st day). In this period, in the blood serum of the experimental rats, a statistically significant increase in the content of total bilirubin level, a rise in the transaminases level, increase in average AF activity, and a decrease in the content of TP and creatinine were observed.
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