SKIN STRUCTURE CHANGES IN CONTACT NICKEL DERMATITIS AT INHIBITION OF INOS
DOI:
https://doi.org/10.11603/1811-2471.2017.v1.i3.8177Keywords:
skin structure, experimental contact allergic dermatitis, nickel, selective inhibitor of iNOS 1400W, nano-capsulated form.Abstract
The search of safe and effective methods of allergic dermatosis’ treatment is an actual problem of modern dermatology. Last decades the role of nitric oxide in body functions is under strict attention and study, in allergic dermatitis also. Use of nanotechnologies has been increasingly raised to treat skin diseases.
Aim of the investigation was to study the structural reorganization of skin in experimental contact nickel dermatitis and its correction with drugs of potent and selective inhibitor of inducible nitric oxide synthase (iNOS) N-([3-(Aminomethyl)phenyl]methyl)ethanimidamide dihydrochloride (1400W) in free and nano-capsulated forms.
Materials and methods. Experiment was carried out on 35 white inbred rats, which were divided into 4 groups. 1st group – intact rats; 2nd – rats with contact nickel dermatitis (CD); 3rd – rats which got free form of 1400W (CD + 1400W); 4th – group which got nano-capsulated form of 1400W (CD + nano-1400W). Polymeric chitosan nanoparticles with encapsulated 1400W were prepared by method of Hussain Z. et al. (2013). 1400W preparations were applicated 2 times per a day during 7 days. The rats were euthanized at 20th day of experiment in accordance to all bioethical principles. Histological examination of skin stripes was performed by routine methods.
Results. Histological examinations of the rat skin with CD showed signs of the most prominent destructive changes at the center of lesion. The epidermis destruction and areas of necrosis were observed. 1400W in free form did not any produced significant changes of skin structure. Application of nano-1400W promoted more active regeneration. Thin layer of epitheliocytes was produced without any differentiation.
Conclusions. Application of nano-encapsulated form of 1400W was more efficient for improving of the skin structure reorganization at contact nickel dermatitis, compared to use of its free form. Reparation of skin was a little bit more active without differentiation of epithelium layers and decreasing of inflammation signs. Further study of combination of nano-1400W with other medications could be prospective.
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