CHARACTERISTICS OF BILE ACID COMPOSITION IN THE BILE OF RATS WITH MONOSODIUM GLUTAMATE-INDUCED OBESITY UNDER CONDITIONS OF CORRECTION WITH NANOCRYSTALLINE CERIUM DIOXIDE
DOI:
https://doi.org/10.11603/1811-2471.2024.v.i2.14723Keywords:
Monosodium glutamate-induced obesity, bile acids, nanocrystalline cerium dioxide, conjugation index, hydroxylation indexAbstract
SUMMARY. The problem of obesity is becoming more and more urgent, which is explained by the increase in the frequency of this disease in connection with excessive and unbalanced nutrition, and a decrease in physical activity.
The aim – to study the effect of nanocrystalline cerium dioxide on the spectrum of bile acids in the bile of rats with simulated glutamate-induced obesity.
Material and Methods. The spectrum of bile acids in the bile of rats with glutamate-induced obesity and under the condition of correction of this pathology with nanocrystalline cerium dioxide was studied using the thin-layer chromatography method. Changes in the conjugation and hydroxylation coefficients of bile acids in half-hourly bile samples obtained during the three-hour experiment were calculated and analyzed.
Results. In rats with monosodium glutamate-induced obesity, a decrease in choleresis, an increase in the concentration of cholic acid, and a probable decrease in the concentration of taurocholic and glycocholic acids were found in all bile samples. The administration of nanocrystalline cerium dioxide to rats leads to increased choleresis, a decrease in the concentration of cholic acid, and a probable increase in the content of glycocholic acid. Nanocrystalline cerium dioxide reduces the concentration of free bile acids in bile, which leads to an increase in the conjugation coefficient relative to the values in a group of animals with glutamate-induced obesity. The hydroxylation coefficient increases due to a decrease in the concentration of dihydroxycholanic acids in bile.
Conclusions. In rats with monosodium glutamate-induced obesity, a decrease in choleresis and an imbalance between the bile acids were found: an increase in the concentration of cholic acid (p<0.05), a decrease in taurocholic acid (p<0.05) and glycocholic acid (p<0.05), a decrease in concentration of total taurochenodeoxycholic and taurodeoxycholic acids (p<0.05). Under the influence of nanocrystalline cerium dioxide in rats with glutamate-induced obesity, choleresis increases, the concentration of cholic acid decreases, the content of glycocholic acid increases (p<0.05), the conjugation coefficient and the hydroxylation coefficient increase.
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