ASSESSMENT OF PROSPECTIVITY OF APPLICATION OF OSTEOINDUСTIVE MATERIALS ACCORDING TO THE DATA OF DENSITOMETRY FOR RECONSTRUCTION OF BONE TISSUE DEFECTS IN DYNAMICS OF THE EXPERIMENT IN THE POSTOPERATIVE PERIOD
DOI:
https://doi.org/10.11603/1811-2471.2020.v.i3.11590Keywords:
osteoplastic material, densitometry, postoperative period, platelet-rich plasma, growth factors, bone regenerationAbstract
Bone tissue is an unknown part of the human body for most medical professionals. Bone mineral density disorder (BMD), according to the World Health Organization, is the fourth most common in the world after cardiovascular disease, cancer and diabetes.
At present, the use of platelet-rich plasma (PRP) is one of the successful areas of tissue engineering and cell therapy in medicine. The use of growth factors is particularly interesting in cases where the effectiveness of bone material and osteointegration is questionable (for example, in severe osteoporosis or scar tissue changes).
The aim of the study – to evaluate the prospects of using osteoinductive materials for the reconstruction of bone defects.
Material and Methods. Osteoplastic properties of the materials were studied in a model of a bone defect of the femur of the diaphyseal zone in Wistar rats, four months old. 4 experimental groups were formed: control group (intact animals) – 12 individuals; Group I – 14 animals, osteoplastic material “mp3 OsteoBiol” was used to heal the bone defect; Group II – 13 animals, filling the bone defect with platelet-rich plasma (PLP); Group III – 15 animals, with bone defect augmentation used the composition “mp3 OsteoBiol + ZTP”. To obtain platelet-rich blood plasma (PRP) from the patient, 30 ml of blood was taken by venipuncture, which was centrifuged at double speed, after which the blood in the test tube was divided into 3 parts. The syringe was used to select the average, viscous mass of yellowish color (PRP). During the studies, local densitometry was performed to measure the optical density of bone tissue. Statistical processing of the obtained results was performed on a personal computer using the licensed programs “Microsoft Excel” and “Statistica”.
Results and Discussion. According to the results of studies after 3 months, it was found that the maximum value of BMD in intact animals did not differ statistically from the same parameter in rats of group III (1199.25±63.70) HU and (1145.0±62.71) HU, respectively, p, p1>0.05, p2<0.01). In this case, in animals of I and II experimental groups, the maximum values of BMD were 1.2 times, p<0.05, and 1.4 times, p1<0.05, respectively, lower relative to the data of this parameter in animals of the control group. The minimum BMD data in rats of the control and III experimental groups were equal to each other (924.19±67.00) HU and (849.26±59.22) HU, p>0.05, p1, p2<0.05, respectively). At the same time, the data of this parameter in rats of I and II experimental groups remained 1.5 times less than the data in animals of the control group, p<0.01, p1>0.05. At the same time, the analysis of the mean BMD data showed that the values of this parameter were equal to each other in the animals of the control and III experimental groups, p>0.05, p1, p2<0.05. At the same time, the mean value of BMD in animals of I and II experimental groups was 1.2 times and 1.3 times, p1>0.05, lower than in animals of the control group, p <0.05.
Conclusions. According to the clinical signs of the postoperative period and densitometric studies, it was found that our proposed composition containing “mp3 OsteoBiol” and platelet-rich blood plasma, accelerated the process of bone regeneration, compared with the groups where the above-mentioned agents were used alone with the formation of a newly formed bone, which had a mature character.
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