EXPERIMENTAL MODEL OF STAPHYLOCOCCUS AUREUS EXOGENOUS ENDOPHTHALMITIS

Authors

  • N. B. Kuryltsiv Filatov Institute of Eye Diseases and Tissue Therapy, National Academy of Medical Sciences of Ukraine, Odesa, Ukraine
  • K. M. Halei Filatov Institute of Eye Diseases and Tissue Therapy, National Academy of Medical Sciences of Ukraine, Odesa, Ukraine

DOI:

https://doi.org/10.11603/2415-8798.2019.1.9990

Keywords:

exogenous endophthalmitis, Staphylococcus aureus, experimental model, inoculation of pathogen

Abstract

Exogenous endophthalmitis develops due to the penetration of the pathogen into the eye. Postoperative endophthalmitis dominates and makes up about 70–80 %, most often the cause of that is aerobic gram-positive bacteria (90 %). Whereas, post-traumatic endophthalmitis makes up about 25 %, in which in 26.9–37.1% of cases etiological factor is Staphylococcus aureus.

The aim of the study – creation of an effective model of exogenous staphylococcus aureus endophthalmitis in experimental animals.

Materials and Methods. The experiment was conducted in 120 chinchilla rabbits, which were divided into 2 groups (60 rabbits or 120 eyes each): the control and the main. In the control group, intravitreal injection of 10.000 microbial bodies of the strain Staphylococcus aureus 3a from the patient's conjunctiva was performed on two eyes. In the main – 150.000 microbial bodies of the daily culture of the museum strain of microorganisms Staphylococcus aureus ATCC 25923F - 49. The clinical picture of the experimental endophthalmitis was evaluated by point system. The degree of inflammatory changes in the eye in each group of the study was determined by the sum of the scores of the averages.

Results and Discussion. In the control group of rabbits, only on the 4th day after infection, mild endophthalmitis was obtained in 104 eyes (86.6 %). The average total number of points of severity of inflammation was (4.3±0.6). In the main group of rabbits, on the 2nd day after the inoculation of the pathogen, endophthalmitis developed in all eyes (100 %), where in 28 eyes (23.3 %) inflammation corresponded to mild severity, in 92 eyes (76.7 %) – severe. In two eyes (1.7 %) an eyeball perforation occurred. The average total number of points of the severity of inflammation was (9.8±1.0).

Conclusions. Obtaining an effective model of exogenous staphylococcal endophthalmitis makes it possible to re-create it for the study of various aspects of the development and treatment of this pathology.

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Published

2019-04-12

How to Cite

Kuryltsiv, N. B., & Halei, K. M. (2019). EXPERIMENTAL MODEL OF STAPHYLOCOCCUS AUREUS EXOGENOUS ENDOPHTHALMITIS. Bulletin of Scientific Research, (1), 112–115. https://doi.org/10.11603/2415-8798.2019.1.9990

Issue

No. 1 (2019)

Section

EXPERIMENTAL RESEARCH

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