SUBMICROSCOPIC CHANGES OF AEROGHEMATICAL BARRIER COMPONENTS OF THE RESPIRATORY DEPARTMENT OF RATS LUNGS IN EARLY TIMES AFTER ACUTE DAMAGE BY HYDROCHLORIC ACID
DOI:
https://doi.org/10.11603/2415-8798.2018.2.9154Keywords:
aerogematic barrier, ultrastructural changes, acute lung injury.Abstract
Submicroscopic studies of the respiratory part of the lungs after 2 and 6 hours after the experimental acute lung injury with hydrochloric acid established adaptive-compensatory and destructive changes in the components of the air-blood barrier.
The aim of the study – to learn submicroscopic changes in the components of the air-blood barrier of the lungs in the early period after acute lung injury.
Materials and Methods. The experiments were carried out on 30 white mature non-linear male rats weighing 200–220 g. The animals were divided into 3 groups: 1 – control group, 2 – hydrochloric acid damage after 2 hours, 3 – hydrochloric acid damage after 6 hour.
Results and Discussion. In an experiment on mature white rats, a study was made of the submicroscopic state of the components of the air-blood barrier in the early periods after acute lung injury. It has been established that adaptive-compensatory and initial destructive changes of the alveolar epithelium and the walls of the hemocapillary take place at 2 o'clock in the experiment. The cytoplasm of respiratory epitheliocytes during this period of the experiment was focal-edematous and enlightened, organelles were destructively altered. For alveolocytes of type I, there was a significant swelling and clarification of the cytoplasm. During this period of the experiment, an increased number of actively phagocytizing macrophages appeared, which acquired a rounded shape, clearly contoured membranes of the cariolema, their invaginations were determined, and in the karyoplasm euchromatin predominated. In alveolocytes of type II, after 6 hours, the progression of destructive changes was established. For which there were peculiarity hypertrophied nuclei with deep invagination of the cariolema, in which there were few nuclear pores, locally expanded perinuclear space. In the edematous cytoplasm, organelles were found to be destructively altered.
Conclusions. Acute damage to the lungs leads to a disruption of the ultrastructural organization of the air-blood barrier. Established adaptive-compensatory processes and signs of destructive changes in the alveolar epithelium and the walls of hemocapillaries, which leads to deterioration of gas-exchange processes in the lungs.
References
Herridge, M.S, Tansey, C.M. & Matthay, M.A. (2011). Functional disability 5 years after acute respiratory distress syndrome N. Engl. J. Med., 364 (14), 1293-1304.
Ware, L.B. & Matthay, M.A. (2000). The acute respiratory distress syndrome N. Engl. J. Med., 342 (1), 1334-1349.
Matthay, M.A. & Zimmerman, G.A. (2005). Acute lung injury and the acute respiratory distress syndrome: four decades of inquiry into pathogenesis and rational management. Am J Respir Cell Mol Biol., 33, 319-327.
Levitt, J.E., Gould, M.K., Ware, L.B. & Matthay, M.A. (2009). Analytic review: the pathogenetic and prognostic value of biologic markers in acute lung injury. J Intensive Care Med., 24, 151-167.
Pugin, J., Verghese, G., Widmer, M.C. & Matthay, M.A. (1999). The alveolar space is the site of intense inflammatory and profibrotic reactions in the early phase of acute respiratory distress syndrome. Crit Care Med., 27, 304-312.
Zemans, R.L., Colgan, S.P. & Downey, G.P. (2009). Transepithelial migration of neutrophils: mechanisms and implications for acute lung injury. Am J Respir Cell Mol Biol., 40, 519-535.
Rubenfeld, G.D., Caldwell, E., Peabody, E., Weaver, J., Martin, D.P., Neff, M., … Hudson, L.D. (2005). Incidence and outcomes of acute lung injury. N Engl J Med., 353, 1685-1693.
Lee, J.W., Fang, X., Dolganov, G., Fremont, R.D., Bastarache, J.A., Ware, L.B. & Matthay, M.A. Acute lung injury edema fluid decreases net fluid transport across human alveolar epithelial type II cells. J Biol Chem, 282, 24109-24119.
Novikov, N.Yu., Tyshkevich, L.V. & Dzhansyz, K.N. (2012). Patomorfologicheskiye izmeneniya aerogematicheskogo baryera pri ostrom respiratornom distress-sindrome v eksperimente (Pathomorphological changes in the air-blood barrier in acute respiratory distress syndrome in an experiment). Tavricheskiy mediko-biologicheskiy vestnik – Taurian medical and biological bulletin, 15, 1 (57), 169-175 [In Russian].
(1986). European convention to the protection of vertebrate animals used for eksperymental and ather scientific purposes. Council of Europe. Strasbourg, 123, 52.
Horalskyy, L.P., Khomych, V.T. & Kononskyy, O.I. (2011). Osnovy histolohichnoi tekhniky i morfofunktsiohalni metody doslidzhen u normi ta pry patolohii (Fundamentals of histological technology and morphofunctional methods of research in norm and pathology). Zhytomyr : Polissya, p. 288 [in Ukrainian].
Beskyy, V.O., Hryshchuk, L.A., Shumeliak, A.M. & Marushchak, M.I. (2018). Features of surfactant biochemical composition in rats with acute respiratory distress syndrome. Medychna ta klinichna khimiia – Medical and Clinical Chemistry, 20 (1), 111-116 [in Ukrainian].
Downloads
Published
How to Cite
Issue
Section
License
Authors who sent their manuscript to "Вісник наукових досліджень. Bulletin of Scientific Research" Surgery agree to the following terms:
a. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal. 
b. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
c. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access)
