CORROSIVE INFLUENCE ON PERIPHERAL OXIDATION OF LIPIDS AND ANTIOXIDANT SYSTEM IN LUNGS IN THE DYNAMICS OF EXPERIMENTAL ALLERGEN ALVEOLITIS IN THE STRESS CONDITIONS

Abstract

Important role in understanding the pathogenesis of allergic alveolitis is played by processes of lipid peroxidation (LPO) and antioxidant system (AOS). Regarding the corrective effect on the violation of the processes of the LPO and the AOS , the great interest of scientists and practitioners is corvitin. In the literature available to us there are no studies related to the study of the effect of corvival on pro- and AOS parameters in the lungs in the dynamics of the development of experimental allergic alveolitis (EAA) under conditions of immobilization stress. Actually, this determines the relevance of our experimental research and points to the expediency of finding new ways of the above correction. The aim of the study – to find out the role of pro- and AOS processes in the pathogenesis of EAA under conditions of immobilization stress in the experiment and to determine the effect of corveton on them.

Materials and Methods. The object of the study – 70 females of guinea pigs, with a body weight of 0.18–0.20 kg, divided into 7 experimental groups, 10 animals in each. The following experimental models were revised: AA before treatment; AA in conditions of immobilization stress before treatment and AA in conditions of immobilization stress after treatment with corvitin. The drug "Corvitin" (Borshchahov chemical and pharmaceutical plant) was administered intradermally at a dose of 40 mg / kg for 10 days. Experimental immobilization stress – by the method of PD Horizon Experimental AA O. O. Oriekhova, Yu. A. Kyrylov. The animals then decapitated and determined  in the lungs the content of the LPO products and the activity of the enzymes AOC. The content of diene conjugates was determined by the method of V. H. Havrylova, VI Mystic-purple, malonic dialdehyde – E. N. Korobainikova, superoxide dismutase activity – by R. Fried method, catalase activity (CT) – by B. Holmes, C. Masters, ceruloplasmin – V. H. Kolb, V. S. Kamyshnikov Statistical analysis by Styuden's method. Statistically reliable results were considered, for which p≤0.05.

Results and Discussion. Studies have shown that the nature of animals with EAA is not the intensification of the processes of LPO (DK and MDA). As a result of the activation of these processes, the lungs accumulated primary and secondary products of LH. The results showed an exhaustion of the AOS (SOD, CT, CP) and the inability to dispose of the products of the LPA. Thus, the study of pro- and AOS parameters in the development of EAA showed an increase in the content of DC and MDA, and decreased activity of enzymes – SOD, CT, CP in lungs for the 2nd, 34th day, indicating the gradual activation of LPA processes against depression of antioxidant defense, especially on the 34th day of the experiment's development. Comprehensive evaluation of the results gives grounds, in our opinion, to conclude that in the conditions of the formation of the EAA there is an imbalance between the POL and the AOS. Taking into account the results obtained, the purpose of the next stage of the work was to identify the imbalance between pro- and AOS at EAA in lungs under stress. The results of the study showed that the experimental model of the disease under conditions of immobilization stress was observed. These results, in our opinion, indicate that the guinea pigs exhausted the activity of AOS, which is unable to fully utilize the products of free radical oxidation of lipids, which are strongly formed under EAA under stress. It can be assumed that the allergy, stress, hypoxia and inflammation of the lungs rapidly lead to the suppression of AOS , the results obtained show that at EAA and stress changes in the parameters of (DK and MDA) and AOS (SOD, CT, CP) in the lungs that manifested by the activation of processes lipoperoxidation against the suppression of AOS. This is especially noticeable on the 2nd, 34th day of the experiment. As a result of the conducted researches, it was found that after 10 days of intrauterine administration of the corvitin product to animals with EAA under conditions of immobilization stress (40 mg / kg), there was a decrease in the content of LPO products and an increase in AOS activity compared with non-exposed animals of this tool. This, in our opinion, indicates corrective action of corvetone on LPO and antioxidant protection in the conditions of EAA and IP formation.

Conclusions. The obtained results of experiments revealed the failure of the antioxidant system to dispose of lipoperoxidation products in experimental allergic alveolitis under stress and its exhaustion at the 2nd, 34th day of the experiment before treatment. The use of anti-oxidant corvitane caused inhibitory effect on the formation of products of peroxidation of lipids and stimulating effect on the activity of the antioxidant system in the lungs, indicating its positive corrective effect of the drug on the disturbed metabolic rate in the formation of experimental allergic alveolitis and immobilization stress.