FEATURES OF IMMUNE HOMEOSTASIS IN THE DYNAMICS OF DEVELOPMENT OF EXPERIMENTAL BRONCHIAL ASTHMA

Authors

  • M. S. Reheda Львівський національний медичний університет ім. Д. Галицького
  • L. A. Lubinets Львівський національний медичний університет ім. Д. Галицького
  • B. F. Shchepanskyi Львівський медичний інститут

DOI:

https://doi.org/10.11603/2415-8798.2018.2.8745

Keywords:

bronchial asthma, immune system, lymphocytes, circulating immune complexes.

Abstract

In this paper, it is shown that the modeling process of bronchial asthma (BA ) is accompanied by changes, in comparison with the control group, in the indicators of immune system response: T- & B-lymphocytes, circulating immune complexes in blood of guinea pig males on the 4th, 18th, 25th day of experiment.

The aim of the study – determination of some indices of immune system in blood of guinea pigs in the modeling process of BA on 4th, 18th, 25th day of the experiment.

Materials and Methods. Experiments were conducted on 40 guinea pigs (males), with body weight 0.25–0.27 kg. Animals were divided into four groups of ten animals in each. Intact guinea pigs were the first group. Animals with experimental BA – the second, third, fourth group respectively on the 4th, 18th, 25th day of the experiment. Experimental BA was reproduced by V. I. Babych method. In blood of intact guinea pigs and animals with experimental BA , the number of T- and B-lymphocytes was determined by the method of E. F. Chernushenko, L. S. Kohosov, determination of the level of circulating immune complexes was carried out by the method of V. Haskova and co-authors. The results of the study were processed by the method of variation statistics using Student's criterion.

Results and Discussion. The results of the studies showed unidirectional changes in individual parameters of the immune system, depending on the periods of the formation of BA : an increase in the number of B-lymphocytes and circulating immune complexes, a decrease in the number of T-lymphocytes for all of the studied days of the experiment.

Conclusions. The obtained results indicate significant changes in the immune system parameters in the blood of experimental animals with BA and are important for understanding the pathogenesis of BA . These studies provide an opportunity to find the more perfect and effective methods of diagnosis of BA.

Author Biographies

M. S. Reheda, Львівський національний медичний університет ім. Д. Галицького

д.мед.н., проф., завідувач кафедрою патофізіології Львівського національного медичного університету імені Данила Галицького

L. A. Lubinets, Львівський національний медичний університет ім. Д. Галицького

к.мед.н., доц. кафедри патофізіології Львівського національного медичного університету імені Данила Галицького

B. F. Shchepanskyi, Львівський медичний інститут

асистент кафедри ортопедичної стоматології Львівського медичного інституту

References

Reheda, M.S., Reheda, M.M., Furdychko, L.O., Kolishetska, M.A. & Myronenko, S.I. (2012). Bronkhialna astma. 5 vyd. [Bronchial astma. 5th ed.]. Lviv [in Ukrainian].

Holgate, S.T. (2012). Innate and adaptive immune responses in asthma. Nature medicine. 18 (3), 673-683. doi:10.1038/nm.2731

Reheda, M.S., Kolishetska, M.A. & Yurevych, V.R. (2015). The influence of thiotriazoline on the change of immune system in the blood of guinea-pigs under the conditions of development of experimental bronchial asthma. Medychna ta klinichna khimiia – Medical and Clinical Chemistry, 17 (2), 52-55 [in Ukrainian]. DOI:http://dx.doi.org/10.11603/mcch.2410-681X.2015.v17.i2.4870

Babych, V.I. (1979). Modyfikatsiya metoda eksperymentalnoi modeli bronkhialnoi astmy u morskykh svynok [Modification of the experimental model of bronchial asthma in guinea pigs]. Problemy patolohyy v eksperymente y klynyke – Problems of pathology in the experiment and in the clinic., 3, 159 [in Russian].

Chernushenko, E.F. & Kohosova, L.S. (1981). Immunolohyia i immunopatolohyia zabolevanyia lehkykh [Immunology and immunopathology of lung disease]. K. : Zdorove.

Haskova, V., Kaslik, J. & Matejckava, M. (1977). Novy zpusob stanoveni circulujicich imunokomplexy wlidskych serech. Cas. Lek. Ces. 116 (14), 436-437.

Published

2018-07-11

How to Cite

Reheda, M. S., Lubinets, L. A., & Shchepanskyi, B. F. (2018). FEATURES OF IMMUNE HOMEOSTASIS IN THE DYNAMICS OF DEVELOPMENT OF EXPERIMENTAL BRONCHIAL ASTHMA. Bulletin of Scientific Research, (2). https://doi.org/10.11603/2415-8798.2018.2.8745

Issue

Section

EXPERIMENTAL RESEARCH