CHANGES IN THYMUS OF ANIMALS OF PROOXIDATIVE AND ANTIOXIDANT SYSTEMS PARAMETERS UNDER THE CONDITIONS OF FORMATION OF EXPERIMENTAL ALLERGIC ALVELOLITIS

Abstract

In this paper it is established that under the conditions of the development of experimental allergic alveolitis at the 34th day of the concentration of malonic aldehyde increases due to the strengthening of lipid peroxidation processes. These changes indicate that in the pathogenesis of the development of experimental allergic alveolitis in guinea pigs an important role is played by the processes of lipid peroxidation and enzyme and non-enzymatic levels of the antioxidant system, as well as the significant depletion of the enzyme chain of the antioxidant glutathione system on the 34th day.

The aim of the study – to learn the state of pro- and antioxidant system in the thymus of animals at different periods of development of experimental allergic alveolitis (EAA).

Materials and Methods. Experiments were conducted on 36 guinea pigs, body weight 0.18-0.20 kg, distributed into four groups (nine animals in each). Intact guinea pigs were in the first group (control). Animals with experimental allergic alveolitis (EAA) – the second, third, and fourth groups, respectively, for 1, 2, and 34 days of the experiment. Determination of tests that reflect the processes of prooxidant (DC, MDA) and antioxidant systems (SOD , catalase) in the thymus were performed in intact guinea pigs and in animals with experimental allergic alveolitis. EAA – by the method of O. O. Orekhov, Yu. A. Kyrylov, 1985. The animals were decapitated under the ethereal anesthesia on the 1st, 2nd, 34th day of the experiment. They conducted a fence of thymus, from which homogenate was prepared. In the homogenate, the activity of catalase (CT) – R. Holmes, C. Masters; superoxide dismutase (SOD) – R. Fried, as well as the content of diene conjugates (DK) by the method of V. B. Havrylov, M. I. Mishkorudna and determined the level of malonic dialdehyde (MDA) by E. N. Korobeinikov method. Statistical processing of digital data was carried out using the Styuden method.

Results and Discussion. The results of research have found that the activity of the enzymes AOS varied unidirectionally, depending on the periods of formation of the experimental AA. Thus, in guinea pigs with experimental AA there is an increase in LPP products both in its initial and final stages. The findings of the studies indicate significant changes in the state of pro- and antioxidant systems in the thymus of animals with exogenous allergic alveolitis and are important for a better understanding of the pathogenesis of EAA. These studies provide an opportunity to find more effective and useful ways to correct the detected changes in EAA.