STATE OF THE IMMUNE SYSTEM IN THE LATE PERIOD OF ULCER FORMATION ON THE BACKGROUND OF EXPERIMENTAL PNEUMONIA AND CORRECTION OF ITS DISTURBANCES BY THIOTRIAZOLIN

Authors

  • L. O. Furdychko Danylo Halytskyi Lviv National Medical University

DOI:

https://doi.org/10.11603/2415-8798.2017.1.7342

Keywords:

pneumonia, gastric ulcer, thiotriazolin.

Abstract

For the pilot study we selected diseases that are common in clinical medicine - pneumonia and gastric ulcer (GU).

The aim of the study is was to establish the immune system in the later period of gastric ulcer and experimental pneumonia (EP) and the impact on affected Thiotriazoline performance.

Materials and Methods. The study was conducted on 49 male guinea pigs, weighing 180–210 g. Experimental pneumonia was caused by the method of  V. N. Shlyapnykov and co-authors, gastric ulcer was simulated by the method of V. I. Komarov. We determined the content of T and B lymphocytes (SD3 and SD19) in the blood by the method of E. F. Chernushenko, L. S. Kohosova; the level of circulating immune complexes (CIC) in blood – by V. Haskova, J. Kaslik. Thiotriazolin was injected intramuscularly at a dose of 100 mg per 1 kg daily from the 10th to the 18th day of the experiment. Animals exercised decapitation under ether anesthesia on the 10th and 18th days of the formation of inflammation in the lungs and stomach ulcers.

Results and Discussion. On the 10th day of the experiment SD19 content was increased on 71.2 % (P<0.05) after 18 days EP and VHSH was increased on 78.9 % (P<0.05) relative to the control group of animals. Research of CIC on the 10th and 18th days of the formation of VC and VHSH – was increased on 86.2 % (P<0,05) and 91.5 % (P<0.05) respectively, compared with intact animals. Content SD3 on the 10th and 18th day was decreased on 68.3 % (P<0.05) and 62.0 % (P<0.05) according to the first group, indicating that depletion of cellular immunity. Application of Thiotriazolin showed an increase of T lymphocytes in the blood to 21.2 % (P<0.05), reduction in lymphocytes in blood and CIC to 26.3 % (P<0,05) and 30.4 % (P<0.05) respectively, compared with the animals on the 18th day of the experiment, which is not exposed to the drug.

Conclusions. Depressed cellular and humoral type of immunity growth was discovered. Use of Thiotriazolin makes a positive impact on cellular and humoral immunity in conditions of EP and GU.

References

Denysiuk, I. V. (2010). Pnevmonii: suchasni standarty diagnostyky ta likuvannia [Pneumonia: current standards for diagnosis and treatment]. Ukrainskyi medychnyi chsopys – Ukrainian Medical Journal, 3 (77), 22–26. [in Ukrainian].

Shliapnikov, V.N., & Solodova, T.L. (1998) Eksperymentalnye movyzvanykh uslovno-patologicheskimi bacteriami I ikh assotsyatsyei: metod. ukazaniia [Experimental models of acute pneumonia caused by conditionally – pathological bacteria and their associations: method. Instructons]. Saratov [in Russian].

Skliarov, O. Ja. (1991). Modelirovanie protsesov gastroprotektsii i ultserogeneza slizovoi obolonki zheludka [Modelling of process of gastroprotection and ulcerogenesis of stomach mucous membrane]. Problemy patologii v eksperimente I klinike – Problems of pathology in the experiment and clinic, XIII, 72–73 [in Russian].

Chernushenko, E.F., & Kogosova, L.S. (1981). Immunologiia i immunopathologiia zabolevanii lenkikh [Immunology and immunopathology of lungs diseases]. Kyiv: Zdorovia [in Russian].

Haskova, V., Kaslik, J., & Matejckava M. (1997). Novy zpusob stanoveni circulujicich imunokomplexy w lidskych serech Cas. Lek. Ces, 116 (14), 436–437.

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Published

2017-04-27

How to Cite

Furdychko, L. O. (2017). STATE OF THE IMMUNE SYSTEM IN THE LATE PERIOD OF ULCER FORMATION ON THE BACKGROUND OF EXPERIMENTAL PNEUMONIA AND CORRECTION OF ITS DISTURBANCES BY THIOTRIAZOLIN. Bulletin of Scientific Research, (1). https://doi.org/10.11603/2415-8798.2017.1.7342

Issue

No. 1 (2017)

Section

EXPERIMENTAL RESEARCH

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