SYNDROME OF ENDOGENOUS INTOXICATION AT EXPERIMENTAL PERITONITIS AGAINST THE BACKGROUND OF DIABETES
DOI:
https://doi.org/10.11603/2415-8798.2019.1.10004Keywords:
acute generalized peritonitis, diabetes mellitus, endogenous intoxication syndromeAbstract
The concept of endogenous intoxication is widely used as a criterion for assessing the body's condition in terms of accumulation of end-products of the metabolism of proteins, lipids and other substances that occurs in various diseases accompanied by increased catabolic processes, in particular in acute common peritonitis on the background of diabetes mellitus.
The aim of the study – to investigate and evaluate the severity of endogenous intoxication syndrome in the experimental animals in the simulated acute common peritonitis on the background of diabetes mellitus compared to animals with experimental acute peritonitis.
Materials and Methods. 56 white rats were used in this work. Diabetes mellitus was modeled by intraperitoneal injection of streptozotocin from Sigmal manufacturer at a rate of 7 mg per 100 g of animal weight, acute peritonitis – by administering 0.5 ml of 10 % captured fecal suspension to the abdominal cavity of the animals under study. Indicators of endogenous intoxication syndrome – medium molecules and erythrocytic index of intoxication. Terms of observation: 1, 3, 7 days from the beginning of the peritonitis modeling. Blood serum and whole blood were used for research.
Results and Discussion. When comparing the level of medium molecules between the study groups of animals, a statistically significant increase in the level of median-molecular peptides during all the experimental periods was established. In particular, the concentration of MM1 and MM2 at the 7th day of the experiment in the study groups was 14.96 % and 54.55 % respectively (p<0.05). This confirms the strengthening of the toxic effects of acute common peritonitis on the background of diabetes mellitus in the test animals. Concerning the degree of damage of erythrocytic membranes between the comparative groups, a similar trend was observed in the level of the erythrocytic index of intoxication for all days of the experiment. Thus, its level in animals of the main group for 1 day since the beginning of the experiment was 1.39 % higher relative to this indicator of the group with acute peritonitis (p>0.05). On the 3rd day this difference was 14.87 %, and on the 7th day – 20.09 %, and it was the largest (p<0.05). Consequently, in animals with experimental acute common peritonitis on the background of diabetes, the manifestations of endogenous intoxication syndrome are more pronounced in comparison with animals with a simulated acute peritonitis.
Conclusions. In animals with acute common peritonitis, on the background of diabetes, manifestations of endogenous intoxication syndrome were more pronounced in comparison with animals with acute peritonitis, which was confirmed by statistically significantly higher levels of medium molecules1, medium molecules2 and erythrocytic index of intoxication (p<0.05).
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