Evaluation of infl uence of osteotropic drugs on activity of lipid peroxidation and antioxidant system processes for bone tissue regeneration in the experiment
DOI:
https://doi.org/10.11603/2311-9624.2018.2.8812Keywords:
biomaterials, regeneration, experiment, malonic dialdehyde, catalase, superoxide dismutase.Abstract
Summary. The comparative estimation of the dynamics of the reparative process in bone tissue under the infl uence of MTA and Nano Gen preparations and the proposed compositions based on hydroxyapatite and β-tricalcium phosphate for endodontic treatment is given.
The aim of the study – to determine the dynamics of reparative processes in bone tissue under the infl uence of drugs and compositions for endodontic treatment, which is based on the analysis of the results of biochemical studies of lipid peroxidation and antioxidant system.
Materials and Methods. The experiment was conducted on 120 white rats, on which the defect of bone tissue was created and fi lled with the studied biomaterials. From the experiment the animals were excised at 14 and 30 days by decapitation under anesthesia and blood samples were taken for biochemical studies. In the blood of rats, the activity of peroxide oxidation processes of lipids – malonic dialdehyde and antioxidant system enzymes – catalase and superoxide dismutase were studied.
Results and Discussion. In animal groups where hydroxyapatite and β-tricalcium phosphate based compositions were used, less activity was observed in lipoperoxidation than in animals using MTA and Nano Gen. The proposed compositions possess antioxidant properties that contribute to the reduction of the activity of the infl ammatory process. Compositions based on hydroxyapatite and β-tricalcium phosphate at earlier times restore the balance in the lipid peroxidation system and the antioxidant system, which creates more favorable conditions for biorevitalization and regeneration of non-apical tissues.
Conclusions. The compositions based on hydroxyapatite and β-tricalcium phosphate possess antioxidant properties, suppress excessive lipoproxidation processes, contribute to the reduction of the activity of the infl ammatory process, which stimulates the regeneration of bone tissue.
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