The features of oral microbiocenosis in rats with periodontitis on the background of hyper- and hypothyroidism
DOI:
https://doi.org/10.11603/2311-9624.2019.2.10400Keywords:
periodontitis, thyroid dysfunction, microbiocenosisAbstract
Summary. The oral cavity is an ecological system that is inhabited by more than 700 species of microorganisms, organized into a biofilm. Phenomena of dysbiosis play an important role in the pathogenesis of periodontitis.
The aim of the study – to carry out a comparative analysis of the frequency of colonization by oral microorganisms in rats of the control group and in animals with periodontitis without comorbidities and against the background of hyper- and hypothyroidism.
Materials and Methods. The experiments were conducted on 48 outbred adult white rats male weighing 180-200 g. Material sampling for microbiological studies (from the surface of teeth on the edge of hard tissue and gums in the interdental spaces) was performed using a standard sterile tampon of the Sarstedit transport system (Germany). Identification of isolated pure cultures was carried out according to morphological, tinctorial, cultural, biochemical properties and signs of pathogenicity.
Results and Discussion. Comparing the species composition and the percentage of excretion of certain types of microorganisms in rats of the control group and animals with simulated periodontitis, the changes in the qualitative composition of microflora, manifested by the appearance of strains of Staph. aureus, E. coli, P. aeruginosa, and yeast-like fungi of the genus Candida albicans. Comparing the species composition and the percentage of excretion of individual microorganisms in rats with simulated periodontitis without concomitant pathology and against the background of thyroid dysfunction, there was no change in the species composition, however, a significantly higher frequency of colonization for strains of Staph. aureus and yeastlike fungi of the genus Candida albicans. At the same time, no significant changes were found between groups with periodontitis on the background of hyper- and hypothyroidism.
Conclusions. Dystrophic-inflammatory process in the rats’ periodontium on the background of thyroid dysfunction is accompanied by an increase in the species number of microorganisms, which is probably a factor in enhancing the inflammatory processes in the periodontium, changes in its functional and antigenic properties, as well as a modulation factor of local and systemic immune reactions.
References
Pavlenkova, E., Pavlenko, S., Tkachenko, I., & Sidorova A. (2018). Kliniko-laboratornoye obosnovaniye ispolzovaniya preparata na osnove antiseptika Troklosene dlya lecheniya patsiyentov s khronicheskim generalizovannym parodontitom II i II stepeni tyazhesti [Clinical and laboratory study of the use of the drug based on the antiseptic Troklosene for the treatment of patients with chronic generalized periodontitis II and III severity]. Wiad Lek, 71(4), 855-860 [in Russian].
Tomilina, T.V. (2014). Razvitiye disbioza v parodonte krys posle splenektomii [Development of dysbiosis in periodontal rats after splenectomy]. Journal of Health Sciences, 04 (01), 125-134 [in Russian].
Toncheva, K.D., Korol, D.M., Kindiy, D.D., Kindiy, V.D., Yarkovoy, V.V., & Korobeynikov, L.S. (2015).
Bioplonki v stomatologii [Biofilms in dentistry]. Stomatologicheskaya nauka i praktika. – Dental science and practice, 5 (10), 36-44 [in Russian].
Fik, V.B. (2015). Mikrobiotsenoz porozhnyny rota piddoslidnykh tvaryn pry tryvalomu vplyvi opioyidnoho analhetyka [Microbiocenosis of the oral cavity of experimental animals with prolonged exposure to opioid analgesics]. Novyny stomatolohiyi. – Dental news, 1 (82), 54-58 [in Ukrainian].
Yelisyeyeva, O.V., & Sokolova, I.I. (2014). Mikroflora rotovoi porozhnyny khvorykh na khronichnyi heneralizovanyi parodontyt na tli chervonoho ploskoho lyshaia [Microflora of the oral cavity of patients with chronic generalized periodontitis on the background of red flat lice]. Visnyk problem biolohiyi i medytsyny – Bulletin of Biology and Medicine, 1 (107), 146-149 [in Ukrainian].
Kramar, V.S., Dmitriyenko, S.V., & Klimova, T.N. (2010). Mikroekologiya polosti rta i yeyo rol v razvitii stomatologicheskikh zabolevaniy: monografiya [Microecology of the oral cavity and its role in the development of dental diseases: monograph]. MZ i SR VolgGMU, 251 [in Russian].
Savelyeva, N.N. (2016). Osobennosti mikrobiotsenoza polosti rta u bolnykh khronicheskim generalizovannym parodontitom I–II stepeni tyazhesti na fone lyamblioza i gelmintozov [Peculiarities of oral microbiocenosis in patients with chronic generalized periodontitis of grades I – II against giardiasis and helminthiasis]. Eksperymentalna i klinichna medytsyna. – Experimental and Clinical Medicine, 3 (72), 127-134 [in Russian].
Shcherbakova, D.S. (2012). Deystviye antiseptikov na bakterialnyye bioplenki u patsiyentov s vospalitel’nymi zabolevaniyami parodonta [The effect of antiseptics on bacterial biofilms in patients with inflammatory periodontal diseases]. Parodontologiya. – Periodontics, 4 (61), 65-69 [in Russian].
Moyseeva, E.H. (2008). Metabolycheskyi homeostaz y imunnaia reaktyvnost orhanyzma v dynamyke vospalenyia v tkaniakh parodonta [Metabolic homeostasis and immune reactivity of the organism in the dynamics of inflammation in periodontal tissues]. Extended abstract of Doctor’s thesis. Sumy: SumSU [in Russian].
Ratushnenko, V.O. (2010). Funktsionalna rol tioldysulfidnoi systemy pry eksperymentalnomu hipo- i hipertyreozi [Functional role of thiol-disulphide system in experimental hypo- and hyperthyroidism]. Odeskyi medychnyi zhurnal – Odessa Medical Journal, 2 (118), 1720 [in Ukrainian].
European convention for the protection of vertebrate animals used for experimental and other scientific purposes. (1986). Council of Europe. Strasbourg, 123, 52.
De Vos, P., Garrity, G.M., Jones, D., Krieg, N.R., Ludwig, W., Rainey, F. A., ..., Whitman, W. B. (2009). Bergeys Manual of Systematic Bacteriology Second Edition Volume Three The Firmicutes. Springer Dordrecht Heidelberg London New York, 1422 p.
