EXPERIMENTAL STUDY OF THE EFFECT OF SALPINGECTOMY ON REDOX PROCESSES AND THE DEVELOPMENT OF INFLAMMATION IN THE OVARIES OF SEXUALLY MATURE FEMALE RATS
DOI:
https://doi.org/10.11603/24116-4944.2025.1.15410Keywords:
rats, salpingectomy; ovary, redox processes, inflammation, superoxide dismutase, catalase, malondialdehyde, acid phosphataseAbstract
The aim of the study – to experimentally determine the effect of salpingectomy on the activity of inflammation, free radical oxidation and antioxidant protection in the ovaries of sexually mature female rats.
Materials and Methods. The experiment included 25 female white Wistar rats aged 4 months. Female rats were randomly divided into five experimental groups. Two groups of 5 intact rats aged 4 months and 7 months, 10 rats that had one fallopian tube removed at the age of 4 months, and 5 rats that had 2 tubes removed at the age of 4 months. Ovaries were removed from intact animals aged 4 and 7 months, as well as from 15 rats with salpingectomy 3 months after the operation. The removed ovaries were homogenized, centrifuged at the rate of 20 mg of tissue per 1 ml of 0.05M Tris-HCl buffer pH 7.5. The content of malondialdehyde, the activity of superoxide dismutase, catalase and acid phosphatase were determined in the supernatant.
Results and Discussion. It was established that age-related changes in the metabolic properties of rat ovaries are characterized by a decrease in the activity of the antioxidant defense system. Three months after unilateral salpingectomy, accumulation of free radical oxidation products and active inflammatory processes were observed in the ovaries on the side of removal of the fallopian tube and in the contralateral ovaries, which were more intense in the ovaries on the side of removal of the fallopian tube. Bilateral salpingectomy, compared with unilateral, led to a greater decrease in the activity of superoxide dismutase and more pronounced inflammatory processes in the tissues of the adjacent ovaries.
Conclusions. Both unilateral and bilateral salpingectomy lead to the development of inflammation and imbalance of free radical oxidation and antioxidant defense systems in the ovaries of sexually mature female rats, which are expected to be more pronounced with bilateral salpingectomy. The development of inflammation is indicated by an increase in the activity of the lysosomal enzyme acid phosphatase in ovarian homogenates. Oxidative stress is manifested by the accumulation of malondialdehyde in ovarian tissue against the background of depletion of superoxide dismutase activity and preservation of catalase activity.
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