EVALUATION OF OXIDATIVE STRESS AND LEVEL OF MOLECULAR DAMAGE IN CHILDREN WITH PNEUMONIA FROM THE IODINE DEFICIENCY REGION
DOI:
https://doi.org/10.11603/24116-4944.2018.1.8802Keywords:
community-acquired pneumonia, intracellular iodine distribution, oxidative stress, cytotoxicity, iodine deficiency.Abstract
The aim of the study ‒ to learn the state of antioxidant protection and the subcellular distribution of iodine in children with moderate and severe course of community-acquired pneumonia.
Materials and Methods. Seventy children with community-acquired pneumonia were examined. All children were examined subcellular iodine in the blood, the state of the antioxidant defense system, the state of stress-responsive systems and the level of molecular damage were assessed.
Results and Discussion. A lower concentration of organified iodine was found (by 12 % and 59 %, respectively) and a high concentration of inorganic iodine (21 % and 48 %, respectively) in moderate and severe course pneumonia in compare to the control group. The severe course of pneumonia was accompanied by activation of catalase, suppression of superoxide dismutase, and against the background of increased oxyradical content, intensive formation of oxidative modification of proteins, and enhancement of DNA fragmentation. The revealed changes tend to decrease depending on the severity of pneumonia.
Conclusions. The distribution of intracellular pool of iodine in the body are directly proportional to the severity of communityacquired pneumonia. The heavy flow of pneumonia contributes to a sharp increase in the concentration of inorganic iodine with a simultaneous decrease in the level of organic iodine in the blood. Fluctuations in the indices of oxidative stress and the level of molecular damage in children with community-acquired pneumonia correlate with the severity of the disease.
References
End preventable deaths: Global Action Plan for Prevention and Control of Pneumonia and Diarrhoea. World Health Organization/The United Nations Children’s Fund (UNICEF) 2013.
Kozlov, V.K. (2016). Vnebolnichnye pnevmonii u detey na sovremennom etape [Community‒acquired pneumonia in children at the present stage]. Habarovsk: OOO Izdatelskiy dom “ARNO” [in Russian].
Reshetar, D.V. (2015). Nehospіtalnі pnevmonіi: deiakі aspekty patohenetychnykh mekhanіzmіv rozvytku [Community acquired pneumonia: some aspects of pathogenic mechanisms]. Naukovyi visnyk Uzhhorodskoho universytetu, seriia “Medytsyna” – Scientific Bulletin of Uzhhorod University, Series “Medicine”, 51 (1), 246-253 [in Ukrainian].
Zimmermann, M.B., & Andersson, M. (2012). Update on iodine status worldwide. Curr. Opin. Endocrinol. Diabetes Obes., 19, 382-387.
Mamenko, M.E. (2017). Profіlaktyka yododefіtsytnykh zakhvoriuvan: shcho maie znaty ta mozhe zrobyty pedіatr і lіkar zahalnoi praktyky? (Klіnіchnі rekomendatsii) [Prevention of iodine deficiency: what should a pediatrician and general practitioner know and be able to do? (Clinical recommendations)]. Sovremennaya pediatriya – Modern Pediatrics, 82 (2), 8-16 [in Ukrainian].
Falfushynska, H.I., Hnatyshyna, L.L., Osadchuk, D.V., Shidlovskyi, V.O., & Stoliar, O.B. (2011). Metalodeponuiucha funktsiia ta anty-oksydantni vlastyvosti shchytopodibnoi zalozy liudei, khvorykh na yododefitsytnyi vuzlovyi koloidnyi zob [Thyroid metaldepositing function and antioxidating properties in patients with iodine-deficit nodular colloid goiter]. Ukr. Biokhi., Zhurn. – Ukrainian Biochemical Journal, 83 (6), 92-97 [in Ukrainian].
Falfushynska, H., Hnatyshyna, L., & Shulhai, A. (2015). Oxidative stress in human thyroid gland under iodine deficiency nodular goiter: from harmless to hazard depending on copper and iodine subcellular distribution. International Journal of Medicine and Medical Research, 1 (1), 5-11.
Yasinskyi, R.M., Raznatovska, O.M., Rastvorov, O.A. (2012). Stan perekysnoho okyslennia bilkiv pry himiorezystentnomu tuberkulozi ta SNID‒asotsiiovanomu tuberkulozi [The state of peroxidation of proteins at drug-resistant tuberculosis and AIDS‒associated tuberculosis]. Ukr. zhurn. kiln. ta med. medytsyny – Ukrainian Journal of Clinical and Laboratory Medicine, 7 (1), 189-193 [in Ukrainian].
Zhiyou, C., & Liang‒Jun, Y. (2013). Protein oxidative modifications: beneficial roles in disease and health. J. Biochem. Pharmacol. Res., 1, 15-26.
Donos, A., Garaeva, S., Leorda, A., Furduj, V., Postolati, G., Redkozubova, G. (2016). Patogeneticheskie mekhanizmy obmennykh narusheniy v usloviyakh vnebolnichnykh pnevmoniy i rekurrentnykh respiratornykh zabolevaniy [Рathogenetic mechanisms of metabolic disorders under community‒acquired pneumonia and recurrent respiratory diseases]. Studia Universitattis Moldaviae, 96 (6), 58-64 [in Russian].
Muravlyova, L., Molotov–Luchankiy, V., Bakirova, R., Klyuyev, D., Demidchik, L., & Lee, V. (2016). Characteristic of the oxidative stress in blood of patients in dependence of community-acquired pneumonia severity. J. Med. Sci., 1 (4), 122-127. doi: 10.3889/oamjms.2016.040
Duong, C., Seow, H.J., Bozinovski, S., Anderson, G.P., Vlahos, R. (2010). Glutation peroxidase‒1 protects against cigarette smoke‒induced lung inflammation in mice. American Journal of Physiology Lung Cellular and Molecular Physiology. 3 (299), 425-433. doi: 10.1152/ajplung.00038.2010.
Balamayooran, G., Batra, S., Balamayooran, M.B., Fessler, K.I., & Jeyaseelan, S. (2010). Mechanisms of neutrophil accumulation in the lungs against bacteri, 43 (1), 5-16.
Raia, P., Parrishc, M., Tay, I.J., Ackerman , A., He, F., Kwang, J., … Engelward, B.P. (2015). Streptococcus pneumoniae secretes hydrogen peroxide leading to DNA damage and apoptosis in lung cells. PNAS, 6, 3421-3430. – Retrieved from: www.pnas.org/cgi/doi/10.1073/pnas.1424144112
Rai, P., Parrishc, M., Tay, I.J., Li, N., Ackerman, S., Kwang, J., … Engelward, B.P. (2015). Streptococcus pneumoniae secretes hydrogen peroxide leading to DNA damage and apoptosis in lung cells. Proc. Natl. Acad. Sci. U S A, 112, 3421-3430.
Wu, M. (2011). Host DNA repair proteins in response to Pseudomonas aeruginosa in lung epithelial cells and in mice. Infect. Immun., 79 (1), 75-87.
Chumduri, C., Gurumurthy, R.K., Zadora, P.K., Mi, Y., & Meyer, T.F. (2013). Chlamydia infection promotes host DNA damage and proliferation but impairs the DNA damage response. Cell Host Microbe, 13 (6), 746-758.
Donos, A., Garaeva, S., Leorda, A. (2015). Profili svobodnykh aminokislot v sivorotke krovi kak pokazatel immunnogo statusa chasto boleyushchikh detey i detey s pnevmoniyey [Profiles of free amino acids in the blood serum as an indicator of the immune status of often ill children and children with pneumonia]. Eurasion Union Science (EUS). Medical sciences, 21 (12), 60-63 [in Russian].
Usonis, V., Ivaskevicius R., Diez‒Domingo J., Esposito S., Falup-Pecurariu, O.G., Finn , A., … Greenberg , D. (2016). Comparison between diagnosis and treatment of community-acquired pneumonia in children in various medical centres across Europe with the United States, United Kingdom and the World Health Organization guidelines. Pneumonia, 8, 5 [in Russian]. https://doi.org/10.1186/s41479-016-0005-y
Nakaz MOZ Ukraini vіd 13.01.05 № 18 Pro zatverdzhennia Protokolіv nadannia medychnoi dopomohy dіtiam za spetsіalnіstiu “Dytiacha pulmonolohіia” [Order of МОH of Ukraine from 13.01.05 № 18 About claim of Protocols of grant of medicare to the children after speciality the "Child's pulmonology"]. Retrieved from: http://www.moz.gov.ua [in Ukrainian].
Maidannyk, V.H., Yemchynska, Ye.O. (2014). Klinichni nastanovy z diahnostyky ta likuvannia pozalikarnianoi pnevmonii u ditei z pozytsii dokazovoi medytsyny [Clinical guidelines for the diagnosis and treatment of community-acquired pneumonia in children from the point of view of evidence-based medicine]. Kyiv [in Ukrainian].
Hassoun, E.A., & Ray, S. (2003). The induction of oxidative stress and cellular death by the drinking water disinfection by-products, dichloroacetate and trichloroacetate in J774 A1 cells. Comp. Biochem. Physiol., 2 (13), 119-128.
Bass, D.A., Parce, J.W., & Dechatelet, L.R. (1993). Flow cytometric studies of oxidative product formation by neutrophils: A graded response to membrane stimulation. Immunol., 130, 1910-1917.
Olive, P.L. (1988). DNA precipitation assay: a rapid and simple method for detecting DNA damage in mammalian cells. Environ. Mol. Mutagen., 4 (11), 487-495.
Shіdlovskyi, V.O., Stoliar, O.B., Osadchuk, D.V. (2009)/ Deklaratsіinyi patent na korysnu model. Method for determining the concentration of iodine in a biosubstrate. UA patent 45332 [in Ukrainian].
Assessment of iodine deficiency diseases and monitoring their elimination: a guide for program managers. ‒ 3rd edition. Kyiv: "K.I.S.".
Wal, S.B., Oh, J.Y., Diers, A.R ., & Landar, A. (2012). Oxidative modification of proteins: an emerging mechanism of cell signaling. Frontiers in Physiology, 3, 369-378.
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