LEVEL OF HORMONES OF STRESS LIMITING SYSTEM OF PREGNANT WOMEN WITH PSYCHO-EMOTIONAL DISORDERS STIPULATED BY ANXIETY
DOI:
https://doi.org/10.11603/24116-4944.2018.1.8624Keywords:
pregnancy, psycho-emotional state, anxiety, hormones, prolactin, β-endorphin, insulin and cortisol.Abstract
The aim of the study – determination of level of hormones of stress limiting system of pregnant women with regard to their psycho-emotional state.
Materials and Methods. There were examined 86 women in II trimester and at the beginning of III trimester of pregnancy. The main group included 60 pregnant women with medium and high level of state anxiety (SA) and 26 pregnant women with anxiety level of 30 points and lower. This fact indicates the low level of SA (comparison group). In order to estimate the state anxiety and trait anxiety (TA) the scale of Spielberger C.D. adapted by Hanin Yu. L. was used. Quantitative assessment of concentration of hormones in blood plasma was made by enzyme immunoassay with SIRIO S device. In order to determine prolactin level (Pr) Monobind INC test system (USA) was used. For cortisol (K) and insulin (In) determination DRG test systems (Germany) were used. Level of endogenous opioid peptides (β-endorphin) was determined with Peninsula Laboratories, LLC test system (USA).
Results and Discussion. Examination groups were divided depending on SA (state anxiety) level. There was determined that average SA index in comparison group made (26.3±1.2) and it was statistically and significantly lower (p<0.05) than the average index in the main group ((37.9±1.4) points). During research of Pr concentration there was stated that mutual influence with anxiety is confirmed by presence of positive correlation between its level and SA (r=+0.690, p<0.05) and also less significant one with TA (trait anxiety) (r=+0.254, p<0.05) correspondingly. During assessment of Pr level there was determined statistically and significantly higher (p<0.05) level among pregnant women with medium-high anxiety level of 136.2 (118.0; 151.6) ng/ml comparing to women with low anxiety level of 93.7 (67.4; 110.7) ng/ml. During comparison of C concentration in pregnant women with medium-high level of SA – 416.0 (368.2; 560.0) ng/ml there was determined statistical and significant (p<0.05) prevalence of this level comparing to women who had low anxiety level– 343.2 (304.6; 370.5) ng/ml. Mutual influence of SA and C level in pregnant women with high and medium levels is also confirmed by available positive correlation (r=+0.704, p<0/05). Such correlations were not revealed in pregnant women with low SA level. During assessment of In level there was determined that it didn’t have correlation with anxiety as well as gestation term and age of pregnant women. Thus, level of In among pregnant women with medium-high anxiety level made 11.5 (9.1; 16.8) μl U/ml and in group of women with low anxiety level it made 12.2 (9.8; 21.9) μl U/ml and it was not statistically significant (p>0.05). However calculation of C/In index as stress marker permitted to determine its statistically significant (p<0.05) prevalence in group of pregnant women with medium-high anxiety level of 39.4 (23.2; 54.9) comparing to women with low anxiety level of 24.6 (16.02; 34.1). During assessment of β-endorphine there wasn’t determined statistically significant difference (p>0.05) between indices of pregnant women with medium-high SA level – 1.2 (0.6; 3.6) ng/ml and low SA level – 1.4 (0.7; 3.0) ng/ml. Probably it indicates absence of obvious influence of specified neuropeptide on formation of emotional state of women during pregnancy.
Conclusions. Assessment of level of prolactin as endogenous anxiolytic permits to determine its statically significant increasing (р<0.05) in pregnant women with medium-high level of SA comparing to group of women with its low level. Increasing of prolactin level in pregnant women with low SA level depends on gestation term (r = +0.665, p<0.05) and it is not connected both with level of SA and TA. The opposite situation occurred among pregnant women with medium and high level of SA: there was negative connection with pregnancy term (r = -0.338, p>0.05) and obvious connection with SA level (r = +0.576, p<0.05). Comparing of level of insulin and β- endorphinin pregnant women with regard to SA level did not permit to determine statistically significant difference (p>0.05). However determination of C/In index as the stress marker permitted to define its statistically significant prevalence (p<0.05) in group of pregnant women with medium-high level comparing to pregnant women who had low level of SA.
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