ALLELIC POLYMORPHISM OF 405G/C LOCUS OF VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF) GENE AMONG WOMEN WITH CIRCULATORY SYSTEM PATHOLOGY.
DOI:
https://doi.org/10.11603/24116-4944.2014.2.5898Keywords:
pregnancy, pathology of blood circulation, vascular endothelial growth factor.Abstract
The objective was to study the distribution of alleles and genotypes of polymorphic locus 405G/C gene VEGF (vascular endothelial growth factor) to develop an informative marker characterizing processes incorporating "angiogenesis" for various diseases, namely, for pregnant women with pathological circulation. Molecular genetic studies of the polymorphic locus 405G/C gene of vascular endothelial growth factor VEGF was performed using the restriction analysis of PCR products corresponding sequences. Examined 20 pregnant women with pathology of the circulatory system and 20 in the control group of pregnant women with uncomplicated somatic history. In women, a significant amount of experimental group -15 (75%) had hypertension Grade II, 2 (10%) of women - thrombophlebitis, 1 woman (5%) - carotid artery aneurysm, the combination of hypertension II Art. and varicose veins, varicose veins and bronchial asthma. In women with the pathology of circulatory system revealed a significant increase in the genotype GG (P > 0.05) polymorphic locus 405G/C VEGF gene compared with healthy pregnant women and tends to reduce the genotype CC (5%) and GC (40%) of the VEGF gene in comparison with healthy pregnant women (15% and 60% respectively). Found that the presence of the genotype G/G polymorphic locus 405G/C VEGF gene increases the risk of disease of the circulatory system in 3.67 times.
References
Zaporozhan V.M. Suchasni pohlyady na diahnostyku hestatsiynykh uskladnenʹ /V. M. Zaporozhan, V. P. Mishchenko, I. V. Rudenko//Zbirnyk naukovykh pratsʹ Asotsiatsiyiakusherivhinekolohiv Ukrayiny. - K.:Intermed. - 2011. - S. 364-369.
Podolʹsʹkyy V.V. Osoblyvosti reproduktyvnoho zdorov*ya zhinok fertylʹnoho viku z shkidlyvymy zvychkamy /V. V. Podolʹsʹkyy, V. L. Dronova, R. S. Teslyuk [ta inshi.] //Zbirnyk naukovykh pratsʹ Asotsiatsiyi akusheriv-hinekolohivUkrayiny. - K.: Intermed. - 2011. - S. 686-689.
Hryshchenko V.O. Puty reshenyya problemy perynatalʹnykh poterʹ / O. V. Hryshchenko, V.V. Bobrytskaya, O. B. Demchenko [ta insh.] //Zbirnyk naukovykh pratsʹ Asotsiatsiyi akusheriv-hinekolohiv Ukrayiny. - K.:Intermed. - 2011. - S. 205209.
Hruzyntseva N.A. Prenatalʹnyy monitorynh ta analiz rannʹoho neonatalʹnoho periodu u novonarodzhenykh z pryrodzhenymy vadamy rozvytku i vysokym ryzykom spadkovoy patolohiyi/N.A.Hruzyntseva, O.YA.Seniv //Tavrycheskyy medyko-byolohycheskyy vestnyk.-2012.-№2,ch.1.-S.90 -93.
HnateykoO.Z. Ekohenetychni aspekty patolohiyi lyudyny, sprychynenoyi vplyvom shkidlyvykh faktoriv zovnishnʹoho seredovyshcha./O.Z. Hnateyko, N.S.Luk'yanenko - "Zdorovʹe rebenka". - 2007. - № 6 (9). - S. 82 - 87.
Zakharova N.B., Durnov D.A., Mykhaylov V.YU., Ponukalyn A.N., Nykytyna V.V., Zankyna O.V., Leonova M.L. Dyahnostycheskoe znachenye yssledovanyya faktora rosta éndotelyya sosudov v syvorotke krovy // Fundamentalʹnye yssledovanyya. - 2011. - № 11 (chastʹ1).str.215-220;
Molekulyarno-henetycheskye narushenyya v hene N1 y metylyrovanye nekotorykh henov - supressorov v sporadycheskykh svetlokletochnykh kartsynomakh pochky / D.S. Mykhay- lenko, M.V. Hryhorʹeva, V.V. Zemlyakova y dr. // Onkourolohyya. - 2010. - №2. - S. 32-36.
Kushlynskyy N.E., Hershteyn E. S. Byolohycheskye markery opukholey v klynyke - dostyzhenyya, problemy, perspektyvy // Molekulyarnaya medytsyna. - 2008. - №3. - S. 48-55.
Kushlynskyy N.E., Hershteyn E.S., Lyubymova N.V. Byolohycheskye markery opukholey: metodycheskye aspekty y klynycheskoe prymenenye // Vestnyk Moskovskoho onkolohycheskoho obshchestva. - 2007. - №1. - S. 5-7.
Amo Y., MasuzawaM., HamadaY., Katsuoka K. Serum concentrations of vascular endotheHal growth factor-D ip angiosarcoma patients // Bntish Journal of Dermatology, 150(1), 2004. - P. 160-161.
Pat. 32044 UA, MPK G01N33/49 (2006.01) Sposib vydilennya DNK z leykotsytiv peryferiynoyi krovi/MakukhH. V., Zastavna D.V., Tyrkus M. YA. [ta in.], zayavnyk DU «Instytut spadkovoyi patolohiyi AMNU». - № u200801896; zayavl. 14.02.2008; opubl. 25.04.2008, Byul. №8.
Mc. Pherson M.J. PCR a Practical Approash. Oxford Unwe^ty press/ Mc M. J. Pherson, P. Qrnrke, G. R. Taylor. - New York: Oxford Urnversrty press, 1993. - 253 p. 22
Assodaton of theVEGFgene polymorphism w^bi diabetic retinopathy IP type 2 diabetes patients / M. Buraczynska, P. ^azek, I. Baranow^z-Gaszczykand L. Jozwiak//Neprology DialysisTransplantation. - Vol.22, №3. - P. 827-832.
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