THE RELATIONSHIPS BETWEEN COMPLAINTS, MELATONIN LEVELS AND SOME INDICATORS OF THE STATE OF THE ANTIOXIDANT SYSTEM AND ANGIOGENESIS IN WOMEN WITH UTERINE LEIOMYOMA
DOI:
https://doi.org/10.11603/24116-4944.2024.1.14658Keywords:
uterine leiomyoma, melatonin, reduced glutathione, VEGF, lipid peroxidation, antioxidant systemAbstract
The aim of the study - to research the relationships between complaints, melatonin levels and some indicators of the state of the antioxidant system and angiogenesis in women diagnosed with uterine leiomyoma.
Materials and Methods. 60 women of reproductive age with a diagnosis of uterine leiomyoma (experimental group) were examined. The control group consisted of 20 healthy women of the same age group. The concentrations of melatonin and reduced glutathione, as well as the levels of vascular endothelial growth factor VEGF in the blood plasma of the examined women were studied. Comparisons between the groups were carried out using the t-test for unequal samples. Calculations of logistic regression between qualitative (presence or absence of those described below clinical symptoms) and quantitative (listed biochemical parameters) parameters was performed.
Results and Discussion. The level of melatonin in the blood plasma of patients with uterine leiomyoma was significantly lower compared to the level of this hormone in healthy women, namely, 111.01 ± 18.5 ng/ml compared to 153.5 ± 8.5 ng/ml in the control group, p<0.01. The plasma concentration of reduced glutathione in women with leiomyoma was significantly lower compared to healthy women (0.77 ± 0.13 μmol/L versus 1.02 ± 0.14 μmol/L in the control group, respectively, p<0.01). The level of VEGF in patients with uterine leiomyoma was significantly higher (90.4 ± 23.6 pg/ml) compared to the control group (35.1 ± 8.3 pg/ml), p<0.01. With a decrease in plasma melatonin levels below 100.0 ng/ml in women of reproductive age, patients with uterine leiomyoma have an 82% chance of painful menstruation, and with an increase in the concentration of reduced glutathione above 0.78 μmol/L, the chance of profuse bleeding during menstruation is approximately 67%. The level of VEGF in the blood plasma of women with leiomyoma above 96.13 pg/ml, according to our data, is almost always associated with the presence of spontaneous miscarriages in the anamnesis of such patients.
Conclusions. 1. In patients with uterine leiomyoma, there is a reduced activity of the antioxidant system compared to healthy women: in the experimental group, the average level of melatonin in the blood plasma was significantly (p<0.01) lower compared to the control group (the difference was 27.6 percent), as well as the average plasma level of reduced glutathione (the difference was 24.5 percent at p<0.01, compared to the control). 2. In the group of women diagnosed with uterine leiomyoma, there was a significant (2.58-fold) increase in the average concentration of VEGF in blood plasma compared to healthy women (p<0.01), which indicates the activation of angiogenesis in this disease. 3. The relationships between the clinical signs of uterine leiomyoma and the studied biochemical parameters are described using logical regression tools.
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