APOPTOSIS OF NEUTROPHILS OF THE SECRETION OF THE CERVICAL CANAL IN PREGNANT WOMEN WITH POST-VIDAL SYNDROME
DOI:
https://doi.org/10.11603/24116-4944.2023.2.14262Keywords:
covid-19, pregnancy, fetal growth retardation, apoptosisAbstract
The aim of our study – to determine the role of neutrophils in the activation of programmed cell death, as a marker of the apoptosis process, and their role in the development of gestational complications during fetal growth retardation in pregnant women with post-covid syndrome.
Materials and Methods. The study of neutrophils in the secretion of the cervical canal was carried out by the method of flow cytofluorimetry. The proportion of neutrophils that were at the stage of apoptosis and necrosis was determined, the correlation of these indicators with the uncomplicated course of the gestational process during the physiological course of pregnancy and with the development of gestational complications during fetal growth retardation in pregnant women against the background of post-covid syndrome. 60 pregnant women (the main group) with SARS-CoV-2, positive Ig A, M or G to SARS-CoV-2 at 30–34 weeks of pregnancy took part in the study. The control group consisted of 25 women with physiological pregnancy.
Results and Discussion. When evaluating the apoptosis of neutrophil granulocytes in the blood and cervical mucus of the subjects examined at the 32nd week of pregnancy, it was found that in women with a singleton pregnancy and the physiological course of the gestational process, the early apoptosis of blood neutrophils was within (4.45 ± 0.25) %, in the cervical mucus – (5.14 ± 0.32) %. In pregnant women of the main group with an uncomplicated course of gestation, the proportion of annexin-positive neutrophils in the blood was (15.80 ± 0.18) %, while in pregnant women of the main group with a complicated course of pregnancy, which was accompanied by fetal growth retardation, the level of apoptosis was (24.30 ± 0.50) % of neutrophil cells, which was significantly different from the indicators of the control group (5.14 ± 0.32) %, (р<0.001). IL-32 concentration was 67.27±5.63 pg/ml in pregnant women of the control group. The post-covid syndrome causes an increase in these indicators in the main group of pregnant women by 2.8 times compared to the control group (188.36±25.22, (p<0.001)
Conclusions. The development of metabolic disorders and apoptotic changes in the placental tissue are confirmed at the morphological level in the form of destructive and necrotic changes in the microcirculatory channel of the placenta. An increase in the level of annexin-positive neutrophils and activation to the degree of neutrophil necrosis accompanies fetoplacental dysfunction and is an important marker for predicting the development of fetal growth retardation in pregnant women with post-covid syndrome.
References
Blitz, M.J., Gerber, R.P., Gulersen, M., Shan, W., Rausch, A.C., Prasannan, L., ... & Rochelson, B. (2021). Preterm birth among women with and without severe acute respiratory syndrome coronavirus 2 infection. Acta Obstetricia et Gynecologica Scandinavica, 100(12), 2253-2259. DOI: https://doi.org/10.1111/aogs.14269
Aabakke, A.J., Petersen, T.G., Wøjdemann, K., Ibsen, M.H., Jonsdottir, F., Rønneberg, E., ... & Bliddal, M. (2023). Risk factors for and pregnancy outcomes after SARS-CoV-2 in pregnancy according to disease severity: a nationwide cohort study with validation of the SARS-CoV-2 diagnosis. Acta Obstetricia et Gynecologica Scandinavica, 102(3), 282-293. DOI: https://doi.org/10.1111/aogs.14512
Aimes, R.T., Zijlstra, A., Hooper, J.D., Ogbourne, S.M., Sit, M.L., Fuchs, S., ... & Antalis, T.M. (2003). Endothelial cell serine proteases expressed during vascular morphogenesis and angiogenesis. Thrombosis and haemostasis, 89(03), 561-572. DOI: https://doi.org/10.1055/s-0037-1613388
Keskin, A., Ustun, G., Aci, R., & Duran, U. (2022). Homocysteine as a marker for predicting disease severity in patients with COVID-19. Biomarkers in Medicine, 16(7), 559-568. DOI: https://doi.org/10.2217/bmm-2021-0688
Menter, T., Mertz, K.D., Jiang, S., Chen, H., Monod, C., Tzankov, A., ... & Bruder, E. (2021). Placental pathology findings during and after SARS-CoV-2 infection: features of villitis and malperfusion. Pathobiology, 88(1), 69-77. DOI: https://doi.org/10.1159/000511324
Ponti, G., Maccaferri, M., Ruini, C., Tomasi, A., & Ozben, T. (2020). Biomarkers associated with COVID-19 disease progression. Critical reviews in clinical laboratory sciences, 57(6), 389-399. DOI: https://doi.org/10.1080/10408363.2020.1770685
Varga, Z., Flammer, A.J., Steiger, P., Haberecker, M., Andermatt, R., Zinkernagel, A.S., ... & Moch, H. (2020). Endothelial cell infection and endotheliitis in COVID-19. The Lancet, 395(10234), 1417-1418. DOI: https://doi.org/10.1016/S0140-6736(20)30937-5
Wong, Y.P., Khong, T.Y., & Tan, G.C. (2021). The effects of COVID-19 on placenta and pregnancy: what do we know so far? Diagnostics, 11(1), 94. DOI: https://doi.org/10.3390/diagnostics11010094
Rizzo, G., Mappa, I., Maqina, P., Bitsadze, V., Khizroeva, J., Makatsarya, A., & D’Antonio, F. (2021). Effect of SARS-CoV-2 infection during the second half of pregnancy on fetal growth and hemodynamics: a prospective study. Acta Obstetricia et Gynecologica Scandinavica, 100(6), 1034-1039. DOI: https://doi.org/10.1111/aogs.14130
Severinsen, E.R., Kähler, L.K., Thomassen, S.E., Varga, T.V., Fich Olsen, L., Hviid, K.V., ... & Nielsen, H.S. (2021). Mental health indicators in pregnant women compared with women in the general population during the coronavirus disease 2019 pandemic in Denmark. Acta obstetricia et gynecologica Scandinavica, 100(11), 2009-2018. DOI: https://doi.org/10.1111/aogs.14258
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