The influence of drinking water with different content of potassium and sodium stearates on free radical processes in the body of rats
DOI:
https://doi.org/10.11603/mcch.2410-681X.2018.v0.i1.8884Keywords:
potassiumstearate, sodiumstearate, lipidperoxidation, antioxidantdefense, TBK-activeproducts, dieneconjugates, catalase, superoxidedismutase.Abstract
Introduction One of the reasons for the lack of quality drinking water is the poor quality of natural water, which is constantly polluted by waste water from industrial and municipal enterprises, surface runoff from the fields and territories of settlements with which it enters a large amount of toxic substances. Among them, surface-active substances such as potassium and sodium stearate are not the last.
The aim of the study to determine the specifics of lipid peroxidation and the state of the antioxidant protection under the influence of potassium and sodium stearates on the body of the experimental rats when they enter with drinking water.
Research Methods. White rats were divided into 7 groups, which consumed water with stearates in quantities equal to the maximum non-active dose of the substance (MND), 1/2 MND and 1/4 MND for 25 days. The content of TBC-active lipid peroxidation products, diene conjugates and the activity of superoxide dismutase and catalase in liver homogenate were determined.
Results and Discussion. In the homogenate of the liver of animals of all groups, there was a significant increase in the level of diene conjugates and TBK-active products in proportion to the concentration of the substance. It was also found that in animals that consumed water with different concentrations of potassium stearate, inhibition of antioxidant enzymes was observed. Consumption of water with different concentrations of sodium stearate caused the activation of these enzymes.
Conclusions. Prolonged use by test animals of aqueous solution with concentrations of potassium and sodium stearate in MND and 1/2 MND negatively affects the state of hepatocyte cell membranes due to the activation of the lipid peroxidation processes, namely TBK-active products and diene conjugates, the amount of which depended on the concentration of stearates in drinking water. The use of drinking water by test animals with maintenance of potassium and sodium stearates at various doses caused changes in the content of antioxidant enzymes: water with potassium stearate inhibits the activity of these indicators, and causes sodium activation with sodium stearate.
References
Prokopov, V.O. (2014). Stan ta yakist pytnoi vody tsentralizovanykh system vodopostachannia Ukrainy v suchasnykh umovakh (pohliad na problemu z pozytsii hihiieny) [Condition and drinking water quality in centralized water supply systems of Ukraine for today (view on the problem with hygiene positions)]. Hihiiena naselenykh mists – Hygiene of Inhabited Areas, 64, 56-67 [in Ukrainian].
Prokopov, V.O., & Lypovetska, O.B. (2012). Otsinka yakosti pytnoi vody z pidzemnykh vododzherel Ukrainy z pohliadu vplyvu na stan zdorovia naselennia [Assessment of the quality of drinking water from underground
water sources in Ukraine in terms of impact on the health of the population]. Naukovyi visnyk NMU – Scientific Journal of NMU, 4, 122-126 [in Ukrainian].
Onishchenko, G.G. (2013). O sanitarno-epidemiologicheskom sostoyanii okruzhayushchey sredy [On sanitary and epidemiological state of the environment]. Gigiyena i sanitariya – Hygiene and Sanitary, 2, 4-10 [in
Russian].
Voloshhenko, O.I., & Mudriy, I.V. (2001). Gigiyenicheskoe znachenie poverkhnostno-aktivnykh veshchestv [Hygienic value of surfactants]. Kiev: Zdorovye [in Russian].
Prodanchuk, M.H., Mudryi, I.V., Hudz, O.V., & Kalashnikov, A.A. (2006). Suchasnyi stan toksykolohohihiienichnoi otsinky poverkhnevo-aktyvnykh rechovyn (ohliad literatury) [Modern status of toxicological and hygienic assessment of superficially active substances (literature review)]. ovremennye problemy toksikologii –Modern Problems of Toxicology, 2, ttp://www.medved.
kiev.ua/arhiv_mg/2_2006.htm [in Ukrainian]
Shvets, V.I., Tymofiichuk, I.R., Semenenko, S.B., Shvets, N.V. (2017). Vplyv poverkhnevo-aktyvnykh rechovyn na orhanizm liudyny [Influence of surface-active substances on the human organism]. Klinichna ta eksperymentalna patolohiia – Clinical and Experimental Pathology, 16, 2 (60), 115-119 [in Ukrainian]
Lototska, O.V. (2012). Vplyv stearatu natriiu na bilkovoutvoriuiuchu funktsiiu v orhanizmi piddoslidnykh tvaryn [Effect of sodium stearate on protein generating function in the body of experimental animals]. Hihiiena
naselenykh mists: zbirnyk nauk. Prats – Hygiene of Inhabited Areas: collection of Scientific Works, 59, 87-91 [in Ukrainian].
Mudrii, І.V. (2001). Toksikoloho-hіhіienіchna otsіnka syntetychnykh poverkhnevo-aktyvnykh rechovyn (ohliad lіteratury) [Toxicological hygienic evaluation of synthetic top-active substances]. Sovremennye problemy
toksikologii – Modern Problems of Toxicology, 3, 55-60 [in Ukrainian].
Kondratiuk, V.A. & Lototska, O.V. (2011). Sanitarno-toksykolohichna otsinka stearatu kaliiu za rezultatamy bilkovoho obminu v eksperymenti [Sanitary toxicological evaluation potassium stearate for the results of protein metabolism in experiment]. Hihiiena naselenykh mists: zbirnyk nauk. Prats 58, 107-111 [in Ukrainian].
Voloshchenko, O.I. (2010). Otsinka imunotoksychnoi dii poverkhnevo-aktyvnykh rechovyn ta enzymiv skladovykh novykh syntetychnykh myinykh zasobiv [Assessment of immunotoxic action of surfactants and enzymes components of new synthetic detergents]. Dovkillia ta zdorovia – Environment and Health, 4, 12-16 [in Ukrainian].
Stalnaya, I.D., & Garishvili, T.G. (1971). Metod opredeleniya malonovogo dialdegida s pomoshchyu tiobarbiturovoy kisloty [Method for determination of
malondialdehyde with thiobarbituric acid using modern methods in iochemistry]. V sb. Sovremennye metody v biokhimii. Moscow: Meditsyna, [in Russian].
Stalnaya, I.D. (1977). Metod opredeleniya diyenovoy konyugatsyi nenasyshchenykh vysshykh zhyrnykh kislot [Method for determination of diene conjugates of higher fatty acids]. Orekhovyche V.N. (Ed.). Moscow:
Meditsyna [in Russian].
Chevary, S., Chaba, I., & Seney, I. (1988). Rol superoksiddismutazy v okislitelnykh protsessakh kletki i metody ee opredeleniya v biologicheskom materiale [The role of superoxide dismutase in oxidative processes of cells and methods of its determination in biological material]. Laboratornoe delo – Laboratory Work, 11, 678-681 [in Russian].
Koroliuk, M.A. (1988). Sposob opredeleniya aktivnosti katalazy [Method for determination of catalase activity]. Laboratornoe delo – Laboratory Work, 1, 6-19 [in Russian].
Rebrova, O.Yu. (2006). Statisticheskiy analiz meditsynskykh dannykh. Primenenie paketa programm Statistica [Statistical analysis of medical data. Application of the Statistica package]. Moscow: MediaSfera [in Russian].
