The study of bas content and hemostatic action of liquid extract of corn silk for the use in obstetrics and ginecology
DOI:
https://doi.org/10.11603/mcch.2410-681X.2018.v0.i1.8871Keywords:
antihemorrhagiceffect, cormsilk, phytochemicalstudy.Abstract
Introduction. Uterine bleeding is an important social and economic problem. Herba drugs are used both in folk and in evidence medicine for atony of the uterus and uterine bleeding. Therefore, the development and research of herbal preparations with a hemostatic effect for use in uterine bleeding is an important issue.
The aim of the study – to conduct a phytochemical study of the liquid extract of corn silk, as well as study its hemostatic effect.
Research Methods. Phytochemical study of qualitative composition and quantitative content of biologically active substances of liquid extract of cron silk was carried out by qualitative reactions, spectrophotometric and titrimetric methods. A study of the hemostatic effect of a liquid extract of corn silk was carried out on outbred, mature white female rats on a model of capillary bleeding from a cut wound of the uterus. The preparation of the comparison was an extract of water pepper.
Results and Discussion. Sugars, glycosides, tannins, flavonoids, coumarins, saponins were identified in the liquid extract of corn silk. It was determined that the sum of polyphenols in the liquid extract of corn silk is (8.0±0.51) %, tannins – (1.4±0.03) %, flavonoids – (2.35±0.07) %, hydroxycinnamic acids – (3.3±0.23) %, organic acids – (3.23±0.46) % by the quantitative methods of investigation. As a result of prophylactic administration of a liquid extract of corn silk to female rats, the duration of capillary bleeding from the cut cervical wound was significantly reduced. The haemostatic activity of the reference preparation was somewhat lower.
Conclusions. The conducted researches give grounds to assert about the prospects and the need for further research of the hemostatic effect of the liquid extract of corn silk.
References
Unifikovanyi klinichnyi protokol pervynnoi, vtorynnoi (spetsialyzovanoi) ta tretynnoi (vysokospetsializovanoi) medychnoi dopomohy. Anomalni matkovi krovotechi. (2016) Zatverzhdeno nakazom MOZ Ukrainy vid 13.04.2016 № 353. 34 [in Ukrainian].
Trends in maternal mortality: 1990 to 2015: estimates by WHO, UNICEF, UNFPA, World Bank Group and the United Nations Population Division. http://www.who.int/reproductivehealth/publications/monitoring/maternal-mortality-2015/en/
Karpiuk U.V. Kyslychenko V. S. (2017). Analiz asortymentu likarskykh zasobiv hemostatychnoi dii [Analysis of assortment of medicinal products of hemostatic action]. Medychna ta klinichna khimiia – Medical and Clinical Chemistry, 19(2), 53-59 [in Ukrainian].
Kompendium: spravochnik lekarstvennykh sredstv on-line. [Compendium: reference book of medicines] http://compendium.com.ua. [in Russian].
Kuldyrkaieva, K.V. (2009). Inherovanyi AVS/VEN/ chastotnyi analiz spozhyvannia hemostatychnykh likarskyh zasobiv v ramkakh rehionalnoho rynku [Integrated frequency analysis of consumption of hemostatic drugs within the regional market]. Ukrainskyi medychnyi almanakh – Ukrainian Medical Almanac, 12 (5), 105-107 [in Ukrainian].
Kyslychenko, V.S., & Karpiuk, U.V. (2015). Pat. Ukrainy na korysnu model № 103779. (51)МПК А61К 36/899 А61Р 7/04. Sposib oderzhannia iolohichno aktyvnoi substantsii roslynnoho pokhodzhennia z krovospynnoiu diieiu dlia vykorystannia v akusherstvi ta henikolohii [Method for obtaining a biologically active substance of plant origin with a hemostatic action for use in obstetrics and genicology], zaiavnyk i patentovlasnyk Natsionalnyi farmatsevtychnyi universytet, Kyslychenko V.S., № 201507266; zaiavl. 20.07.2015; opubl.25.12.2015, Bul. №24.
(1990). Gosudarstvennaya farmakopeya SSS R. Obshchie metody analiza. Lekarstvennoe rastitelnoe syrye [State Pharmacopoeia of the USS R: Vol. 2. General methods of analysis. Medicinal plant raw materials]. Moscow: Medicine [in Russian].
Derzhavna Farmakopeia Ukrainy. 1-e vyd. Dopovnennia 2. (2008). Kharkiv: Derzhavne pidpryyiemstvo “Naukovo-ekspertnyi farmakopeynyi tsentr” [State Pharmacopoeia of Ukraine / State-owned enterprise "Scientific Expert Pharmacopoeia Center". – The first edition. – Supplemented 2]. [in Ukrainian].
Burda N. Ie. , Zhuravel I. O., Kyslychenko V. S., & Diemokhin, V. B. (2010). Kilkisne vyznachennia orhanichnykh kyslot u travi Filipendula ulmaria (L.) Maxim. [Quantitative determination of organic acids in the her of Filipendula ulmaria (L.) Maxim.]. Ukrainskyi biofarmatsevtychnyi zhurnal – Ukrainian Biopharmaceutical Journal, 1 (6), 59-61 [in Ukrainian].
Karpiuk U., Kotova E., Kotov A., & Kyslychenko V. (2017) Development of method for quantitative analysis of corn silk for inclusion in the draft national monograph of the State Pharmacopoeia of Ukraine. ScienceRise: Pharmaceutical Science, 4(8), 4-7.
(2014). Derzhavna Farmakopeia Ukrainy. 2 vyd. T 3. [State harmacopoeia of Ukraine. 2nd edition. Vol. 3]. Kharkiv: Derzhavne pidpryyemstvo “Naukovo-ekspertnyi farmakopeynyi tsentr”, 358.
Kozhemiakіn, Yu.M., Hromov, O.S., Fіlonenko, M.A., & Saifetdіnova, H.A. (2002). Naukovo-praktychnі rekomendatsіi z utrymannia laboratornykh tvarynta roboti z nymy [Scientific and practical recommendations for the maintenance of laboratory animals and work with them]. Derzh. farmakol. tsentr. Kyiv: Vid. dіm "Avіtsena" [in Ukrainian].
Boyarintsev, V.V., Nazarov V.B., & Fronchek, E.V. (2010). Doklinicheskaya otsenka effektivnosti mestnikh gemostaticheskikh preparatov ksperimentalnoe issledovanie) [Preclinical evaluation of the effectiveness of local hemostatic drugs (experimental study)]. Meditsina katastrof – Disaster Medicine, 3, 24-26 [in Russian].
Fisenko V.P. (Ed.). (2000). Rukovodstvo po eksperimentalnomu (doklinicheskomu) izucheniyu novikh farmakologicheskikh veschestv [Guide to experimental (preclinical) study of new pharmacologic substances]. Moscow: Remedium [in Russian].
Lukianova, L.V. (2011). Metodychnyi pidkhid do vykorystannia aboratornykh tvaryn v umovakh modeli – kapiliarnoi krovotechi z rizanoi rany matky u shchuriv [Methodical approach to the use of laboratory animals under model conditions – capillary hemorrhage from cut uterine wound in rats]. Aktualni problemy suchasnoi medytsyny – Actual Problems of Modern Medicine, 4 (36), 2, 106-109 [in Ukrainian].
Lukianova, L.V. (2007). Informatsiinyi lyst pro novovvedennia №80-2007 “Metodyka opredeleniya dlitelnosti krovotecheniya na modeli rezanoy rany matki u krys” [Newsletter on innovation No. 80-2007 “Method of determination of bleeding duration on models of uterine cutaneous uterine wound in rats”]. Kyiv [in Ukrainian].
