Standardization of phaseolus vulgaris pods dry extract on the content of flavonoids
DOI:
https://doi.org/10.11603/mcch.2410-681X.2018.v0.i1.8786Keywords:
bean leaflets, dry extract, flavonoids, standardization.Abstract
Introduction. The development of new drugs for treatment and prevention of diabetes is a challenge that is an urgent matter in the context of the increase of diabetes morbidity. The hypoglycemic effect of phaseolus vulgaris pods infusion is established. The development of technology of phaseolus vulgaris pods dry extract, the study of its hypoglycemic effect is possible only by determination of the composition of the extract and its standardization.
The aim of the study – to develop methods of phaseolus vulgaris pods dry extract identification; to develop and validate the technique of quantitative determination of flavonoids; to advance the quality criteria of the extract.
Research Methods. Qualitative composition of flavonoids was investigated by means of chromatographic methods; quantitative determination was conducted by spectrophotometric technique.
Results and Discussion. The research of phenolic compounds of phaseolus vulgaris pods dry extract by chromatographic methods allowed identifying rutin, isoquercitrin, ferulic acid and quercitrin in trace amounts. In addition to the phenolic compounds, the extract contained a large quantity of an unidentified flavonoid, a zone of which on the thin-layer chromatogram was situated between the zones of chlorogenic acid and rutin; two hydroxycinnamic acids, the zones of which were below and above the ferulic acid, and probably were their derivatives. The analysis of electronic spectra of absorption of a standard rutin sample solution and a dry extract test solution within the conditions of quantitative determination with aluminum chloride allowed using rutin as a standard for evaluation of flavonoids content. The content of flavonoids was determined by the spectrophotometric method, it was 0.7-0.8% range expressed in rutin and dry substance for different series of the extract.
Сonclusions. To identify the dry extract of phaseolus vulgaris pods it was suggested to choose rutin, isoquercitrin and ferulic acid as active markers. The scheme of the chromatogram, which should be corresponded to the chromatogram of the test solution with the identification of dry extract, was recommended. The content of flavonoids was advised as a quantitative criterion of quality: not less than 0.65% expressed in rutin and dry substance.
References
(2015). Derzhavna Farmakopeia Ukrainy: v 3 t. [State Pharmacopeia of Ukraine]. Kyiv: Ukrayinskyi naukovyi farmakopeinyi tsentr yakosti likarskykh zasobiv [in Ukrainian].
Kohlmünzer, S. (2007). Farmakognozja. Warszawa.
Kyznetsova, M.Yu., Makieieva, O.M., Lavrovska D.O., Tymoshenko, M.O., Sheverova, D.P., Halenova, T.I., … Ostapchenko, L.I. (2015). Effect of aqueous extract from phaseolus vulgaris pods on lipid peroxidation and antioxidant enzymes activity in the liver and kidney of diabetic rats. J. of Applied Pharmaceutical Science, 5(05). Electronic resource: ww.japsonline.com.
Fantini, N., Cabras, C., Lobina, C., Colombo, G., Gessa, G. L., Riva A. … Mauro A. M. Carai (2009). Reducing effect of a phaseolus vulgaris dry extract on food intake, body weight, and glycemia in rats. J. Agric. Food Chem., 57, 9316-9323.
Yurchenko, A., Raksha, N., & Savchuk, O. (2016). The influence of kidney beans (Phaseolus vulgaris) pods extract on obesity development. Visn. Kyyiv. Nats. un-tu im. Tarasa Shevchenka (seriya biolohiia) – Journal of Taras Shevchenko Kyiv National University, 2 (72), 86-8.
Luca, C.D., Olatunde, A., Tijjani, H., & Olisa-Enewe, I. A. (2013). Effect of aqueous extract of Phaseolus Vulgaris L. (Red Kidney Beans) on alloxan-induced diabetic wistar rats. Int. J. of Science Invention Today, 2 (4), 292-301.
Oonsivilai Ratchadaporn, Jutarat Manatwiyangkool, Anant Oonsivilai. (2011). Extraction condition of phaseolus vulgaris. International Journal of Nutrition and Food Engineering, 12 (12), 869-872.
Zou Y.P., & Chang Sam K.C. (2014). Antioxidant and proliferative properties of extract and fractions from small red bean (Phaseolus Vulgaris L.). J. Food and Nutrition, 1 (105), 11.
Kovalov, V.M., Kovalov, S.V., Demeshko, O.V., Dmytriievskyi, D.I., Kutsanian, A.S., Maloshtan, L.M. inventors; Kovalov, S.V., assignee. Sposib oderzhannia kompleksu biolohichno aktyvnykh rechovyn z hipohlikemichnoiu
diieiu UA 101850 (Patent) 2006 Jan 10 [in Ukrainian].
Tundis, R., Loizzo, M. R., Mennichini, F. (2001). Natural products as α-amylase and α-glucosidase inhibitors and their hypoglycaemic potential in the treatment of diabetes: an update. Mini-Reviews in Medical Chemistry,
(4), 315-331.
Barret, M.L., & Udani, Jay K. (2011). A proprietary alpha-amylase inhibitor from white bean (Phaseolus vulgaris): a review of clinical studies on weight loss and glycemic control. Nutrition Journal, 10 (24). doi. org:10.1186/1475-2891-10-24
Vronska, L.V. (2013). Doslidzhennia zi standartyzatsii stulok plodiv kvasoli za vmistom flavonoidiv [Study of the standardization of valves fruit bean on flavonoid content]. Farmatsevtychnyi chasopys, 4, 82-87 [in Ukrainian].
Vronska, L.V., Chubka, M.B., & Demyd, A.Ye. (2015). Development of phenolic compounds chromatographic identification in bilberry shoots. Farmatsevtychnyi chasopys, 3, 28-33.
Lin, Long-Ze, Harnly, J.M., Pastor-Corrales, M.S., & Luthria, D.L. (2014). The polyphenolic profiles of common bean (Phaseolus vulgaris L.). Food Chem., 107 (1),399-410.
Kumar Ganesan, & Baojun Xu. (2017). Polyphenolrich dry common beans (Phaseolus vulgaris L.) and their health benefits. International J. of Molecular Sciences, 18, 26. Electronic resource: www.mdpi.com/journal/ijms
