The investigation of Anchusa officinalis L.carbohydrates
DOI:
https://doi.org/10.11603/mcch.2410-681X.2018.v0.i2.8563Keywords:
Anchusa officinalis L., polysaccharides, monosaccharides.Abstract
Introduction. The research of new plant raw materials is a promising direction in a modern pharmaceutical science. Anchusa officinalis L. contains a wide range of biologically active substances. The plenty of modern experimental studies of Anchusa officinalis L. pharmacological activity and the experience of its use in the folk medicine indicate a necessity of its detailed investigation.
The aim of the study – to explore the carbohydrates of Anchusa officinalis L., quantify polysaccharides’ fractions content and define its monomer composition. Research Methods. The gravimetric method was used to quantify polysaccharides. Qualitative composition of carbohydrates was studied using methods of paper and thin-layer chromatography.
Results and Discussion. The results of analytical reactions indicate the presence of free sugars and watersoluble polysaccharides in the investigated samples of Anchusa officinalis L. The amount of polysaccharides in Anchusa officinalis L. harvested from different places vary because of the influence of plant growth conditions on the accumulation of biologically active substances. The highest amount of polysaccharides fractions was recorded for hemicellulose A. Free sugars (D-glucose and D-fructose) were identified in the Anchusa officinalis L. raw material. In the polysaccharide fractions the neutral and acidic sugars were identified: D-glucose, D-fructose, L-rhamnose, D-xylose, L-arabinose and D-galacturonic acid.
Conclusions. Free sugars and polysaccharides in Anchusa officinalis L. were revealed. The herb contains 6.57–9.39 % polysaccharides, and the roots – 0.67–0.78 %, depending on the place of growth and the year of harvesting. Water-soluble polysaccharides, pectin substances, hemicelluloses A and B were obtained from the raw material of Anchusa officinalis L. The highest rates were recorded for the hemicellulose A – 32.29 % and 12.26 % in the herb and roots, respectively. The qualitative composition of free sugars and fractions of polysaccharides was determined.
References
Hrytsyk, A.R. & Svirska, S.P. (2017). Pryroda likuie… Roslyny rodu Volovyk: botanichna kharakterystyka, sklad ta farmakolohichna diia [Nature treats ... Anchusa genus plants: botanical characteristics, composition and pharmacological activity]. Ivano-Frankivsk: Vydavets Kushnir H.M. [in Ukrainian].
Hilger, H., Selvi, F., Papini, A., & Bigazzi, M. (2004). Molecular systematics of Boraginaceae tribe Boragineae based on ITS1 and TRNL sequences, with special reference to Anchusa L. Annals of Botany, 94, 201-212.
Dovidnyk nazv roslyn Ukrainy [Directory of Plant Names of Ukraine]. Retrieved from: http://ekontsh.civicua. org/system.php?lang=ua [in Ukrainian].
Mosyakin, S.L. & Fedoronchuk, M.M. (1999). Vascular plants of Ukraine: a nomenclatural checklist. Kyiv: National Academy of Sciences of Ukraine, M. G. Kholodny Institute of Botany.
The Plant List. Version 1.1. Retrieved from: http:// www.theplantlist.org/tpl1.1/search?q=Anchusa
Hrodzinskyi, A.M. (Ed.). (1992). Likarski roslyny: Entsyklopedychnyi dovidnyk [Medicinal plants: an encyclopedic guide]. Kyiv: URE im. M.P. Bazhana [in Ukrainian].
Kovalova, V.M. & Marchyshyn, S.M. (2014). Praktykum z identyfikatsii likarskoi roslynnoi syrovyny [The workshop on identification of medicinal plant material]. Ternopil: TDMU [in Ukrainian].
Derzhavna Farmakopeia Ukrainy [State Pharmacopoeia of Ukraine]. (2014). (2th ed., Vol. 3). Kharkiv: DP “Ukrainskyi naukovyi farmakopeinyi tsentr yakosti likarskykh zasobiv [in Ukrainian].
Kyslychenko, V.S., Novosel, O.M. & Abuiusef, A. (2001). Vyvchennia polisakharydiv plodovo-yahidnykh roslyn – yabluni domashnoi ta vynohradu kulturnoho [Investigation of fruit-berry plants polysaccharides – Malus domestica L. and Vitis vinifera L.]. Fiziolohichno aktyvni rechovyny – Physiologically Active Substances, 1 (31), 70-73 [in Ukrainian].
Derzhavna Farmakopeia Ukrainy [State Pharmacopoeia of Ukraine]. (2014). (2th ed., Vol. 1). Kharkiv: DP “Ukrainskyi naukovyi farmakopeinyi tsentr yakosti likarskykh zasobiv [in Ukrainian].
