Interdependence of the pro- and antioxidant status and kallikrein-kinin system in animals of different age in conditions of early and late polytrauma

Abstract

Introduction. The last decades are characterized by significant growth of injuries, especially in young people, which have a variety of causes. One of the pathogenetic links of traumatic disease is a violation of prooxidant-antioxidant and kallikrein-kinin systems. Data on changes in the indicators of the system of lipid peroxidation –antioxidant defense –kallikrein-kinin system are disparate and contradictory in people of all ages and at different periods of traumatic disease.

The aim of the study–to establish interdependence between pro- and antioxidant system and general and specific proteolysis parameters in rats of different ages in early and late display of polytrauma.

Research methods. Simulation of polytrauma was performed under the thiopental sodium anesthetizing (60 mg per kilogram of weight). In aseptic conditions, the animals were caused bleeding from the femoral vein (about 20% of the volume of circulating blood), 1 ml of which was injected into a paranephrine cellulose to reproduce endogenous intoxication. Then from the operative access by the forceps of Luer, the left astragalus was broken, a wound on the thigh was sewn. The research was conducted after 1, 3, 24 hours and 14 days after the polytraumatic simulation. The activity of superoxide dismutase in blood serum was determined by Chiavari's method. The activity of catalase in the liver homogenate and blood plasma was determined by the method of M.A. Koroliuk and co-authors. The content of ceruloplasmin in blood serum was determined according to the method of V. H. Kolba, V.S. Kamyshnikov. The content of TBA-active products was determined according to the method of B.V. Havrylov, and indicators of kallikrein-kinin system– by K. M. Veremieienko. The analysis of the interdependency between the two features in the presence of a normal distribution was estimated following the results of the Pearson’s correlation analysis (r).

Results and Discussion. Having analyzed the interdependency between the pro- and antioxidant system and the indicators of the general and specific proteolysis in the early stages of traumatic disease in animals 3 months of age, we established the presence of a statistically significant inverse correlation between the level of kallikrein and the activity of superoxide dismutase (r=-0.69; p<0.05) for one hour after the polytraumatic simulation. The activation of nonspecific proteolysis was confirmed by the presence of direct statistically significant correlation bonds between the level of proteolytic activity and the level of malondialdehyde (r=0.65; p<0.05) for 1 hour and between proteolytic activity and the level of lipids hydroperoxides for 3 hours of traumatic disease (r=0.67; p<0.05). The decrease in the protective mechanisms of the antioxidant defense system confirmed a significant inverse correlation between proteolytic activity and the catalase of blood in the early stages of polytrauma (r=-0.67;p<0.05) in animals 3 months of age. In the reproductive rats 6 months of age for 1 hour after polytraumatic simulation, fixed inverse statistically significant correlation relations between the level of kallikrein, blood catalase and ceruloplasmin of blood serum (r=0.89 and r=0.72, respectively; p<0.05). For 3^rdhour of the experiment, the existence of inverse correlation relationships between the level of precalicine and the activity of the liver catalase (r=-0.80; p<0.05) was established, which proves the decrease of protective resources of antioxidant defense system with the activation of kallikrein-kinin system.

Conclusion.Detected dependencies confirm that increase of the intensification of lipid peroxidation processes, the activation of general and specific kallikrein-kinin system proteolysis, and the reduction of adaptive resources of antioxidant defense system leads to an increase in lipid disequilibrium in animals of different age groups with polytrauma and vice versa, the increase of lipid status disorders leads to an increase in disequilibrium in the system of lipid peroxidation – antioxidant defense –kallikrein-kinin system.