ACTIVITY OF OXIDATIVE PROCESSES IN RATS WITH ADRENALINE CARDIOPATHY ON THE BACKGROUND OF POISONING WITH THE FOOD COLORANT AZORUBINE
DOI:
https://doi.org/10.11603/mcch.2410-681X.2026.i1.15948Keywords:
azorubine; adrenaline; oxidative stress; antioxidant system; white rats.Abstract
Introduction. Nowadays, when stressful situations arise in the country every day, leading to the development of cardiovascular diseases, there are people who consume products with a large number of food additives, in particular dyes, attracting them with their rich color, which can cause negative reactions in the body. The aim of the study was to determine the activity of prooxidant processes and the antioxidant system in rats with adrenaline cardiopathy on the background of poisoning with the food dye azorubine. Research methods. The experiment used 78 white male rats. The dye E122 was administered intragastrically for 21 days at a dose of 100 mg/kg body weight. Adrenaline was administered intramuscularly once at a dose of 0.5 mg/kg. Euthanasia was performed using sodium thiopental at the 3rd, 24th and 48th hour after adrenaline administration against the background of azorubine poisoning after 7, 14 and 21 days. The content of lipoperoxidation products and oxidative modification of proteins, as well as the content of ceruloplasmin and catalase activity were determined in the blood serum, heart and liver. Results and discussion. After adrenaline exposure in rats poisoned with azorubine, the content of TBA- AP increased in all organs studied and by the end of the experiment in the blood serum exceeded the norm by 7.7 times and was 1.2 times higher than in rats poisoned only with azorubine. A similar increase in this indicator was noted in the liver and heart of rats. After exposure of animals to both toxicants, a significant increase in the content of 2,4-DNFH of both fractions in the blood serum, liver and heart of rats was observed. These indicators progressively increased with the extension of the study period. In the blood serum, the content of ceruloplasmin and catalase activity significantly (p≤0.05) increased, in contrast to the decrease in the latter in the liver and heart of rats affected by both toxicants. Conclusions. Poisoning of rats for 21 days with the food dye azorubine leads to an increase in the content of indicators of lipoperoxidation and oxidative modification of proteins, which becomes even more pronounced after the rats are affected by increased doses of adrenaline. An increase in the content of ceruloplasmin and catalase activity in the blood serum of rats after damage by adrenaline against the background of poisoning with azorubine was noted. During the experiment, catalase activity progressively decreased in the liver and heart of rats affected by both toxicants.
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Accepted 2026-03-09
Published 2026-04-28
