FEATURES OF OXIDATIVE STRESS DEVELOPMENT IN THE LIVER, KIDNEYS, AND LUNGS UNDER CONDITIONS OF POLYTRAUMA COMPLICATED BY SPLENECTOMY
DOI:
https://doi.org/10.11603/mcch.2410-681X.2026.i1.15933Keywords:
multiple trauma; splenectomy; laparotomy; oxidative stress; liver; kidneys; lungs; experiment.Abstract
Introduction. In modern conditions, there has been a significant increase in the level of trauma in Ukraine and worldwide. The background of multiple trauma combined with abdominal trauma, in about 40 % of cases, damage to the spleen occurs. The long-term consequences of splenectomy are well studied today. The effect of spleen removal on systemic disorders in conditions of multiple trauma, in the dynamics of early and late traumatic disease has not been sufficiently studied. The aim of the study is to determine the features of OS development in the liver, kidneys, and lungs under conditions of multiple trauma complicated by splenectomy. Research methods. Experiments were performed on mature male Wistar line rats. In the control group, rats were administered anesthesia. In control group 2, laparotomy was modelled under anaesthesia. In experimental group 1, multiple trauma was modelled. In experimental group 2, rats with multiple trauma underwent additional splenectomy. After 1, 7, 14, and 28 days, the catalase activity and the content of reagents to thiobarbituric acid were determined in a 10 % extract of liver, kidney, and lung homogenate. The antioxidant-prooxidant index (API) was calculated based on their ratio. Results and discussion. Spleenectomy against the background of simulated multiple trauma contributes to an even greater reduction in API, which was statistically significant in the liver from the first day of the post- traumatic period, and in the kidneys and lungs from the seventh day. Splenectomy is accompanied by greater prooxidative disturbances in the liver than in the kidneys and lungs. The degree of reduction in API in the liver during this period was significantly greater than in the kidneys and lungs. Conclusion. Additional splenectomy against the background of multiple trauma significantly exacerbates systemic disorders of traumatic disease, in particular, exacerbates OS in internal organs, which should be taken into account in clinical practice in situations where the spleen has to be removed from the injured patient.
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Accepted 2026-03-06
Published 2026-04-28
