STRUCTURAL RECONSTRUCTION OF THE MUSCLE OF THE URINARY BLADDER AND CHANGES IN ITS ENERGY SUPPLYING IN THE CONDITIONS AT POSTRESECTION PORTAL HYPERTENSION

Abstract

Introduction. The features of energy supply to the muscle of the urinary bladder in hemodynamic disorders in the hepatic portal vein system have not been sufficiently studied. The aim of the study – to study the features of the energy supply of the muscle of the urinary bladder in conditions of postresection portal hypertension. Research Methods. Morphological and biochemical methods were used to study the muscle of the urinary bladder of three groups of Vietnamese pigs: group 1 – 5 control animals, group 2 – 11 pigs with postresection portal hypertension, group 3 – 6 animals with a combination of postresection portal hypertension with multiple organ failure. Postresection portal hypertension was modeled by resection of the left and right lateral lobes of the liver. The content of ATP, ADP, AMP and the ATP/ADP ratio were determined in the muscle of the urinary bladder. Morphometrically, on micropreparations of the muscle, its thickness, relative volume of myocytes, stroma, stromal-myocyte ratios, nuclear-cytoplasmic ratios in myocytes, and the volume of damaged myocytes were determined. Correlation analysis was performed between biochemical and morphometric parameters. Quantitative values were processed statistically. Results and Discussion. It was found that resection of the left and right lateral lobes of the liver in Vietnamese pigs led to the occurrence of postresection portal hypertension. In this case, 35.3 % developed multiple organ failure. Under the conditions of simulated pathology, the thickness of the muscle of the urinary bladder decreased, the relative volume of the stroma, stromal-myocyte ratios, the volume of damaged myocytes increased, and the concentrations of ATP decreased. ADP, AMP, the ATP/ADP ratio and nuclear-cytoplasmic ratios in myocytes were disturbed. The detected changes dominated when postresection portal hypertension was combined with multiple organ failure. The degree of deterioration of the energy supply of the muscular membrane of the urinary bladder correlated with the severity of morphological changes. Conclusions. Removal of the right and left lateral lobes of the liver leads to postresection portal hypertension, significant deterioration of the energy supply of the muscle of the bladder, damage to its structural components. The degree of reduction in the energy supply of the muscle of the urinary bladder prevailed in the combination of postresection portal hypertension with multiple organ failure and correlated with the severity of structural changes.