STUDY OF ANTI-INFLAMMATORY PROPERTIES OF EXTRACTS FROM THE ROOTS OF GENTIANA ASCLEPIADEA L.
DOI:
https://doi.org/10.11603/mcch.2410-681X.2023.i4.14283Keywords:
Gentiana asclepiadea L., liquid and thick extracts, anti-inflammatory activity, antiexudative activity, experimental animals, white outbred rats, non-steroidal anti-inflammatory drugsAbstract
Introduction. The leading place among drugs used to weaken the inflammatory and painful reaction of the body is occupied by non-steroidal anti-inflammatory drugs. But drugs of this group show serious side effects that limit their use. Today, the search and development of new drugs, including herbal origin, with anti-inflammatory and analgesic properties is carried out in order to potentiate therapeutic effects and increase their safety.
The aim of the study – to investigate the anti-inflammatory activity of thick and liquid extracts of Gentiana asсlepiadea roots.
Research Methods. The study of the anti-inflammatory activity of thick and liquid extracts of Gentiana asсlepiadea L. was carried out according to the methodological recommendations of the State Expert Center of the Ministry of Health of Ukraine on sexually mature white outbred rats weighing 180–220 g on a model of aseptic formalin inflammation. The study and analysis of the obtained results were carried out in comparison with the drug from the group of non-steroidal anti-inflammatory drugs – sodium diclofenac. The inflammatory response was assessed oncometrically by increasing the volume of rat paw, which was measured prior to administration of 2 % formalin solution, 1 hour, 3 hours, 5 hours and 24 hours after administration of the phlogogenic agent.
Results and Discussion. The results of experimental studies indicate that the anti-inflammatory activity of the studied extracts was already manifested at 1 hour of the experiment. At 5 hours of the experiment, the antiexudative activity of the test extracts was maximum. After 24 hours from the onset of inflammation, the effectiveness of the use of extracts of Gentiana asсlepiadea decreased slightly. It was found that a thick extract showed a better anti-inflammatory effect.
Conclusions. Anti-inflammatory activity of of thick and liquid extracts of Gentiana asсlepiadea roots in the model of formalin edema was studied. The test extracts at a dose of 100 mg/kg animal body weight were found to exhibit moderate anti-inflammatory activity.
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