THE CHANGES OF BLOOD SERUM PROTEOLYTIC ACTIVITY IN RATS WITH MODELED SECONDHAND TOBACCO SMOKING COMBINED WITH PROLONGED ADMINISTRATION OF MONOSODIUM GLUTAMATE IN THE AGE ASPECT

Abstract

Introduction. Every year tobacco smoking kills more that 8 million people all over the world; 1.2 of them are secondhand smokers. Meanwhile, the characteristic feature of modern food technologies is the usage of food supplements which are not always safe for human’s health.

The aim of the study – to investigate the changes of proteolytic activity of blood serum in rats in case of passive tobacco smoking combined with prolonged administration of monosodium glutamate in the age aspect.

Research Methods. Experiments were held on 32 outbred mature white male-rats 180–200 g weight and 32 outbred immature white male-rats 60–80 g weight. Each group of animals was divided into four subgroups: I – control (n=8); ІІ – rats with modeled secondhand tobacco smoking (n=8); ІІІ – rats, which were injected with monosodium glutamate (n=8); IV – rats with modeled secondhand tobacco smoking combined with the monosodium glutamate injection (n=8). General proteolytic activity of blood serum was identified by using azosubstrates (azoalbumine, azocasein, azocollagen) of the Simko Ltd. Firm (Ukraine), according to the manufacturer’s instructions.

Results and Discussion. On the condition of secondhand tobacco smoking in mature rats’ blood serum the number of decomposition products of azoalbumin, azocasein, azocollagen raises (on 59.8 % (p=0,002), on 38.3 % (p<0.001), on 32.6 % (p=0.005) accordingly), that shows the increased degradation of both high molecular and low molecular proteins and collagen. Secondhand tobacco smoking on the background of MSG usage doesn’t cause possible changes relatively isolated influence of tobacco smoke. Secondhand tobacco smoking on the background of MSG usage in immature rats’ blood serum caused significant changes of azoalbumin lysis, which exceeds data on the condition of isolated influence of tobacco smoke on 40.9 % (p=0.002) and azocasein lysis, which exceeds data on the condition of isolated influence of tobacco smoke on 36.5 % (p=0.003). According to the age aspect, the intensity of changes of azoalbumin, azocasein and azocollagen lysis on the condition of secondhand tobacco smoking on the background of introduction of MSG prevails on 31.38 and 12.7  % accordingly in immature rates.

Conclusion. The received results testify about higher intensity of proteolytic processes in immature rats’ blood in comparison of mature animals. This is possibly caused by higher intensity of the formation of free radicals, lower speed of their neutralization and formation of greater number of modified oxidized proteins.