INFLUENCE OF RESECTION OF DIFFERENT VOLUMES OF LIVER PARENCHYMA ON ITS BILE-FORMING FUNCTION
DOI:
https://doi.org/10.11603/mcch.2410-681X.2019.v.i3.10559Keywords:
liver resection, bile formation, bile excretionAbstract
Introduction. Liver resection is widely performed in clinics today. Removal of large amounts of liver leads to various postresection complications and to changes of bile formation.
The aim of the study – to learn the peculiarities of bile-forming function of liver in resection of various volumes of its parenchyma.
Research Methods. The research was conducted on 45 male lab rats, which were divided into 3 groups. Group 1 consisted of 15 intact animals, group 2 – 15 rats after resection of 31.5 % of liver parenchyma, group 3 – 15 animals after removal of 58.1 % of the volume of liver. In 1 month after the beginning of the experiment under thiopental anesthesia, the common bile duct was catheterised and the bile was taken for 1 hour. Intensity of bile secretion was calculated, concentration of bile acids: taurocholic, glycocholic, cholic, chenodeoxycholic + deoxycholic, content of phospholipids, free fatty acids, triglycerides and proteins were determined. Euthanasia of experimental animals was carried out via bloodletting under thiopental anesthesia in a month from the beginning of the experiment. Histologic micronutrients of the liver were studied light-optically. Quantitative indicators were processed statistically.
Results and Discussion. Resection of 58.1 % of liver parenchyma led to postresection portal hypertension, which is characterized by enlargement of the hepatic portal vein, plethora with dilatation of mesentery veins, venous vessels of the small and large intestines, ascites, splenomegaly. The intensity of bile secretion decreased by 28.9 % compared to the control, the concentration of taurocholic acid decreased by 21.5 %, glycocholic – by 15.56 %, cholic – by 18.6 %, and chenodeoxycholic+deoxycholic – by 16.2 %, the concentration of phospholipids decreased by 11.0 %, free fatty acids – by 10.4 %, triglycerides – by 19.8 %, and proteins – by 8.02 %. Light-optically there was a plethora of venous vessels, areas of hepatocytes hypertrophy and dystrophy, the appearance of lymphoid-hystiocytic infiltrates and increased stromal structures in micropreparations of the liver in group 3.
Conclusion. Resection of the left and right lateral lobes of liver leads to postresaction portal hypertension and changes in bile formation and bile excretion, which is characterized by a decrease in the intensity of bile secretion, decrease in the amount of bile acids, phospholipids, free fatty acids, triglycerides and proteins.
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