CHANGES IN THE PROCESSES OF ENERGY PROVIDING OF NEUTROPHILS IN RATS WITH PERIODONTITIS ON THE BACKGROUND OF HYPER- AND HYPOTHYROIDISM
DOI:
https://doi.org/10.11603/mcch.2410-681X.2019.v.i3.10556Keywords:
periodontitis, energy-supplying oxidation, thyroid dysfunctionAbstract
Introduction. Inflammatory periodontal diseases are one of the most urgent problems of dentistry that have social significance. Along with the known concepts of their pathogenesis, considerable attention is paid to the activation of peroxide oxidation of lipids, which may interfere with the oxidation of substrates with dehydrogenases and the transport of electrons along the respiratory chain, causing the separation of respiration and oxidative phosphorylation.
The aim of the study – to investigate the processes of energy providing of neutrophils in rats with periodontitis without comorbidities and against the background of hyper- and hypothyroidism.
Research Methods. The study was performed on white male rats, in which periodontitis, periodontitis on the background of hyperthyroidism and periodontitis on the background of hypothyroidism were modeled. In the mitochondrial fraction of the neutrophils population, the succinate dehydrogenase and cytochrome oxidase activity were determined. The number of blood neutrophils with reduced transmembrane mitochondrial potential was determined by flow cytometry using a set of MitoScreen reagents (BD Pharmigen, USA).
Results and Discussion. Succinate dehydrogenase activity in mitochondria of neutrophils of rats with simulated periodontitis decreased by 16.3 % (p<0.001), with simulated periodontitis on the background of hyperthyroidism – by 40 % (p<0.001), with a periodontitis simulated with hypothyroidism – by 26.7 % (p<0.001) vs the index of the control group. Concerning end-stage enzyme of the respiratory chain of mitochondria – cytochrome oxidase, under the condition of periodontitis without concomitant pathology, the activity of enzyme in mitochondria of rat neutrophils significantly did not change. Experimental periodontitis on the background of hyperthyroidism is accompanied by a decrease in cytochrome oxidase activity by 15.2 % (p<0.01), and in hypothyroidic rats – by 16.2 % (p<0.001) vs the control group. Investigation of mitochondrial transmembrane potential (ΔΨm) of blood neutrophils showed that in rats with simulated periodontitis, the percentage of blood neutrophils with reduced ΔΨm increased by 51.6 % (p<0.001), with simulated periodontitis in the background of hyperthyroidism – 2.4 times (p<0.001), with the simulated periodontitis in the hypothyroidism background – 1.8 times (p<0.001) vs the control group.
Conclusion. Modeling periodontitis against a background of thyroid dysfunction is accompanied by a possible inhibition of processes of energy-supplying oxidation, as indicated by decreasing succinate dehydrogenase and cytochrome oxidase activity in mitochondria of blood neutrophils, both under conditions of hyperthyroidism and hypothyroid conditions.
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