THE INFLUENCE OF CELLULAR INJURY OF DIFFERENT SEVERITY, COMPLICATED BY BLOOD LOSS, ON ANTIOXIDANT-PROOXIDANT BALANCE OF THE CORTICAL LAYER OF A KIDNEY

Abstract

The aim is to find out the features of the antioxidant-prooxidant state of the cortical layer of the kidney in conditions of skeletal trauma of varying severity, complicated by blood loss.

Materials and Methods. Experiments were performed on 98 non-linear white male rats weighing 180–200 g, which were in the standard vivarium diet. All animals were divided into 4 groups: control and three experimental ones. In the first experimental group under anesthesia by thiopental sodium simulated skeletal injury by applying dosed impact on the hip, which caused a closed fracture, the second – in addition simulated blood loss of 20–22 % of total blood volume with autoblood introduction into the cavity of the abdomen at a rate of 0.5 ml per 100 g of animal. The rats were withdrawn from the experiment in conditions of anxiety after 1, 3 and 7 days after the simulation of injuries by the method of total blood loss from the heart. In the cortical layer of the kidneys of test animals we tested TBA-active products of lipid peroxidation, catalase activity and antioxidant-expected prooxidant code: catalase / TBA-active products of lipid peroxidation

Results. Given the isolated skeletal injuries causing lipid peroxidation intensity increases to 3 days, and then reduced to 7 days, indicating adequate antioxidant support. Additional adjacent thigh injury and blood loss significantly impairs metabolism in the cortical layer of kidneys, accompanied by a marked shift of antioxidant-prooxidant balance towards oxidative mechanisms of domination and most pronounced after 3–7 days of post-traumatic period. Amid growing content of secondary products of lipid peroxidation in kidney cortical layer in these terms marked by a gradual decrease catalase activity, indicating exhaustion of enzymatic link of antioxidant effect summation confirms the negative impact of skeletal and blood loss.

Conclusion. Simulation of severe skeletal trauma, complicated by blood loss, leads to a shift in the antioxidant-prooxidant ratio in the kidney cortical layer in the direction of the predominance of prooxidant mechanisms, manifested by a significant increase in the content of secondary lipid peroxidation products and decreased activity of catalase that increase from the first to seventh days of the post-traumatic period.