2-MERCAPTOBENZОTHIAZOLE RADIOPROTECTIVE EFFECT ON IN VITRO CELL CULTURE
DOI:
https://doi.org/10.11603/1811-2471.2018.v0.i2.8914Keywords:
ionizingradiation, radioprotectors, cell culture, proliferation, mitosis, differentiation of myogeniccells, apoptosis.Abstract
Aim: to study the radioprotective properties of 2-merсaptobenzothiazole on proliferating cell culture test system.
Material and Methods. Cytological, statistics.
Results. Upon the incubation of inoculated cellline L929 with 2-merсaptobenzothiazole in the concentration range 0.03–3.00 µg/mL statistically significant change (p≤0.05) of cell population density in mono layer cultures was not found. At the same time for all the applied reagent concentrations stimulation of mitotic activity in the terminal (5th day) stage of cultivation was observed. Exposure of cells by gamma-quanta of 60Co in doses of 1, 5 and 10 Gyled to dose-dependant morphological changes in cell culture. The irradiation of cells in the presence of 2-mercaptobenzothiazole significantly reduced the negative effect of radiation on the indicators of vitality of cells and their differentiation in culture.
Conclusions. Quantitative evaluation of radioprotective properties of 2-mercaptobenzothiazole in the test systems of culture of myogenic cells as well as in the L929 line (fibroblast cells) revealed that the highest coefficients of protection (0.31–0.36) and maximum level of factor of dose reduction (4) were observed at 3 mg/ml reagent concentration (irradiation dose – 1 Gy). According to the literature data and our research results, 2-mercaptobenzothiazole might be considered as a reagent with radioactive properties for cells in vitro.
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