INFLUENCE OF CORVITIN ON HUMORAL IMMUNITY INDICES IN EXPERIMENTAL PNEUMONIA DEVELOPMENT
DOI:
https://doi.org/10.11603/1811-2471.2018.v0.i2.8471Keywords:
corvitin, pneumonia, immune system.Abstract
Specific immune response is very important for lung protection against pathogens, which underwent frustrated phagocytosis (mycobacteria, fungi, viruses) and extracellular organisms, which are rather resistant to phagocytosis (incapsulated bacteria). Microbial infections, which avoid natural protective mechanisms, produce a significant number of antigens, leading to the development of antigen-specific immune response. In particular, antigen-specific proliferation and differentiation of B-lymphocytes are the examples.
Currently, bioflavonoids are often used as an accompanying therapy to correct disturbances, induced by long-lasting inflammatory process. Flavonoids possess antioxidant, membrane-protective, angioprotective, anti-inflammatory, immunomodulating properties, etc.
The aim of the study – to investigate the influence of Corvitin on indices of cell-mediated and humoral immunity in experimental pneumonia development (EP).
Material and Methods. Experimental investigation was performed on 30 guinea pigs (males) weighing 180–220 g, divided into 2 groups, 15 animals in each:
I group – guinea pigs with EP on the 20th day;
II group – guinea pigs under the action of corvitin.
This antioxidant was introduced in the dose 40 mg/kg intraperitoneally for 7 days (from 13 to 20 day).
Experimental pneumonia was induced by intranasal introduction of Staphylococcus aureus culture to animals by the method of V. N. Shliapnikov and co-authors. The animals were decapitated on the 20th day of EP development. Amount of T- and B-lymphocytes in the blood was determined by the method of E. F. Chernushenko, L. S. Kohosova. The level of circulating immune complexes was determined by the method of V. Haskova, J. Kaslik.
The obtained results were statistically evaluated by Student’s t-criterion.
Results. Certain principles were detected after performed therapy. In particular, levels of B-lymphocytes and CIC reliably decreased by 26.34 % and 17.54 % (p<0.05), respectively, compared with the indices of guinea pigs on the 20th day without treatment. Thus, introduction of Corvitin medication had a preventive effect in relation to further formation of immune response imbalance and confirmed its anti-inflammatory and immunomodulating effects.
Conclusions. Corvitin prevented further development of immune system imbalance, limiting inflammatory response. This effect may be caused by anti-inflammatory and immune-modulating properties of Corvitin which may be recommended as an accompanying therapy in pneumonia.
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