COMPARATIVE CHARACTERISTIC OF EFFICIENCY OF FREE AND NANO-ENCAPSULATED BETAMETHASONE IN CONTACT NICKEL DERMATITIS
DOI:
https://doi.org/10.11603/1811-2471.2017.v0.i4.8429Keywords:
contact allergic dermatitis, nanotechnology, oxidative stress, treatment, betamethasone dipropionate.Abstract
Topical glucocorticoids (TGCSs) are the drugs of choice for local treatment of contact allergic dermatitis (CD) because of potent anti-inflammatory and anti-allergic properties. But they do not prevent the relapses and have some serious side effects.
The aim of the study is an investigation of efficacy of free and nano-encapsulated betamethasone in contact nickel dermatitis.
Materials and Methods. Experiment was carried out on 35 white inbred rats, which were divided into 4 groups. 1st group – intact rats; 2nd – rats with contact nickel dermatitis (CD); 3rd – rats which got free form of Betamethasone (CD + Betamethasone); 4th – group which got nano-capsulated Betamethasone (CD + nano- Betamethasone). Visual scale and thickness of skin fold were used for estimation of visible changes, also the intensity of oxidative and nitrooxidative stress was studied.
Results and discussion. Application of 5% nickel sulfate caused prominent damage to skin and rising of oxidative and nitrooxidative stress with increased levels of lipoperoxidation products and simultaneous suppression of antioxidant defense indices. Free betamethasone improved the visible changes of the skin; the thickness of skin fold was less by 32.1%. Intensity of oxidative and nitrooxidative stress decreased with positive dynamics of all indices (significant decreasing of TBARS-products, NOx was observed with normalization of activity and content of enzyme and non-enzyme chains of sell protect – SOD, catalase, restored glutathione).
Application of nano-encapsulated Betamethasone was more efficient that it’s free form for visual signs and influence on other indices. It means that further investigation and clinical implementation of nanotechnologies is prospective direction of contact dermatitis treatment.
References
Kuznetsova, Yu. (2015). Kontaktnyy dermatit kak rezultat agressii okruzhayushchey sredy. Chem lechit? [Contact dermatitis as a result of environmental aggression. Than to treat?]. Rossiyskiy allergologicheskiy zhurnal – Russian Allergological Journal, 5, 76-81 [in Russian].
Brasch, J., Becker, D., Aberer, W., Bircher, A., Kränke, B., Jung, K. et al. (2014). Guideline contact dermatitis. Allergo Journal International, 23 (4), 126-138.
Agner, T., Andersen, K.E., Brandao, F.M., Bruynzeel, D.P., Bruze, M., Frosch, P. et al. (2008). Hand eczema severity and quality of life: a cross-sectional, multicentre study of hand eczema patients. Contact Dermatitis, 59 (1), 43-47.
. Soloshenko, E.M., Volkoslavskaya, V.M., Gutnev, O.L. (2014). Dynamika rozpovsiudzhenosti ta zakhvoriuvanosti na poshyreni dermatozy v Ukraini i Kharkivskomu rehioni za ostanni 10 rokiv [The dynamics of the prevalence and incidence of common dermatoses in Ukraine and the Kharkiv region over the past 10 years]. Dermatolohiia ta venerolohiia – Dermatology and Venereology, (1), 68-77 [in Ukrainian].
Lim, H.W., Collins, S.A., Resneck, J.S., Bolognia, J.L., Hodge, J.A., Rohrer, T.A. et al. (2017). The burden of skin disease in the United States. Journal of the American Academy of Dermatology, 76 (5), 958-972.
Ukhanova, O.P., Shishalova, T.N., Kopylov, A.V., Getman, R.A., Lysogora, L.V. (2012). Patomorfologicheskiye izmeneniya kozhi u bolnykh allergicheskim kontaktnym dermatitom pri vozdeystvii nizkomolekulyarnykh allergenov (gaptenov) [Pathomorphological changes in skin in patients with allergic contact dermatitis when exposed to low molecular weight allergens (haptens)]. Meditsinskiy vestnik Severnogo Kavkaza – Medical Gazette of the North Caucasus, 26 (2) [in Russian].
Schalock, P.C., Dunnick, C.A., Nedorost, S., Brod, B., Warshaw, E., Mowad, C. (2017). American Contact Dermatitis Society Core Allergen Series: 2017 Update. Dermatitis, 28(2), 141-143.
Churilov, A.V., Solovyova, E.A., Kozlov, K.P. (2011). Nikel v biosredakh zdorovykh zhenshchin reproduktivnogo vozrasta [Nickel in the biomedia of healthy women of reproductive age]. Ukrainskyi medychnyi almanakh – Ukrainian Medical Almanac, 14 (3), 194-195 [in Russian].
Khan, M.S., Rani, Z., Ahmed, M.L., Hussain, I., Kazmi, A.H. (2016). Evaluation and pattern of nickel dermatitis in patients with allergic contact dermatitis. Journal of Pakistan Association of Dermatology, 15 (2), 136-139.
Raap, U., Stiesch, M., Reh, H., Kapp, A., Werfel, T. (2009). Investigation of contact allergy to dental metals in 206 patients. Contact Dermatitis, 60 (6), 339-343.
Caplan, A., Fett, N., Rosenbach, M., Werth, V.P., & Micheletti, R.G. (2017). Prevention and management of glucocorticoid-induced side effects: A comprehensive review: Infectious complications and vaccination recommendations. Journal of the American Academy of Dermatology, 76 (2), 191-198.
Hajar, T., Leshem, Y.A., Hanifin, J.M., Nedorost, S.T., Lio, P.A., Paller, A.S. et al. (2015). A systematic review of topical corticosteroid withdrawal (“steroid addiction”) in patients with atopic dermatitis and other dermatoses. Journal of the American Academy of Dermatology, 72 (3), 541-549.
Okayama, Y. (2005). Oxidative stress in allergic and inflammatory skin diseases. Current Drug Targets-Inflammation & Allergy, 4 (4), 517-519.
Byamba, D., Kim, T.G., Kim, D.H., Je, J.H., Lee, M.G. (2010). The roles of reactive oxygen species produced by contact allergens and irritants in monocyte-derived dendritic cells. Annals of Dermatology, 22 (3), 269-278.
Schieber, M., & Chandel, N.S. (2014). ROS function in redox signaling and oxidative stress. Current Biology, 24 (10), 453-R462.
Hussain, Z., Katas, H., Amin, M.C.I.M., Kumolosasi, E., Buang, F., Sahudin, S. (2013). Self-assembled polymeric nanoparticles for percutaneous co-delivery of hydrocortisone/hydroxytyrosol: an ex vivo and in vivo study using an NC/Nga mouse model. International Journal of Pharmaceutics, 444 (1), 109-119.
Volkovoy, V.A., Kuchynska, I.V., Tyupka, T.I. (2010). Vyvchennia likuvalnoi efektyvnosti novoyi mazi z lipofilnym kompleksom iz kory osyky “Tremulin” za umov nealerhichnoho kontaktnoho dermatytu v shchuriv [Study of the therapeutic efficacy of a new ointment with a lipophilic complex from the bark of the "Tremulin" aspen under non-allergic contact dermatitis in rats]. Farmakolohiia ta likarska toksykolohiia – Pharmacology and Drug Toxicology, 6, 7-12 [in Ukrainian].
Saito, M., Arakaki, R., Yamada, A., Tsunematsu, T., Kudo, Y., & Ishimaru, N. (2016). Molecular mechanisms of nickel allergy. International Journal of Molecular Sciences, 17 (2), 202.
Brasch, J., Becker, D., Aberer, W., Bircher, A., Kränke, B., Jung, K. et al. (2014). Guideline contact dermatitis. Allergo Journal International, 23 (4), 126-138.
Luss, L.V. (2009). Sovremennyye problemy diagnostiki i terapii allergicheskogo kontaktnogo dermatita [Modern problems of diagnostics and therapy of allergic contact dermatitis]. Dermatologiya – Dermatology, (2), 63-67 [in Ukrainian].
Usatine, R.P., & Riojas, M. (2010). Diagnosis and management of contact dermatitis. American Family Physician, 82 (3), 249-255.
Jacob, S.E., & Castanedo-Tardan, M.P. (2007). Pharmacotherapy for allergic contact dermatitis. Expert Opinion on Pharmacotherapy, 8 (16), 2757-2774.
