SUPERROXIDDYSMUTASE AND CATALASE ACTIVITY IN THE IN THE SMALL INTESTINE WALL, HEART AND LIVER IN EXPERIMENTAL INTAKE OF CARRAGEENAN
DOI:
https://doi.org/10.11603/1811-2471.2017.v0.i4.8378Keywords:
carrageenan, superoxide dismutase, catalase, tissue, experiment.Abstract
Introduction. One of the leading mechanisms of organ dysfunction syndrome formation in various pathologies is the hyperproduction of active forms of oxygen, which triggers the processes of peroxide oxidation in organs and tissues far from the focal point of injury. Occurrence of oxidative stress is also caused by a decrease in the effectiveness of antioxidant protection.
Therefore, the purpose of our study was to determine the activity of superoxide dismutase and catalase in the tissues of the small intestine, myocardium and liver of experimental animals using 1% carrageenan solution.
Materials and methods of research. The study was carried out on 24 white non-linear male rats. The animals of the experimental group were provided with free access to a 1.0% of carrageenan solution for 1 month. In the selected samples of the small intestine, heart and liver was evaluated the activity of superoxide dismutase and catalase.
Results and discussion. It was established that the use of 1% solution of κ-carrageenan in drinking water caused the activation of the enzyme level of the antioxidant defense system. An increase in the activity of SOD in the wall homogenate of the small intestine (by 1,32 times, p<0,05) and in the liver (1,30 times, p<0,05) was observed. The activity of catalase in the wall of the small intestine homogenate (by 1,18 times, p<0,05) and in the liver (1,13 times, p<0,05) also increased. At the same time, the coefficient of antioxidant protection in the intestine wall was 1.11 (p<0.05), in the myocardium - 1.0 and in the liver - 1.15 (p<0.05).
Conclusion. Oral administration of a 1% carrageenan solution in experimental animals leads to statistically significant activation of the enzyme level of antioxidant defense in the wall of the small intestine and liver, which is characterized by an increase, respectively, in the activity of SOD (31.8% and 29.7%) and catalase (18.3% and 12.9%), p <0.05.
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