CHANGES OF PERIPHIC OXIDES OF LIPIDS IN SUPPLY AT EXTRUSION OF EXTRUSION OF ALLERGIC ALLEVOLITIS IN STRESS CONDITIONS
DOI:
https://doi.org/10.11603/1811-2471.2017.v0.i4.8349Keywords:
exogenous allergic alveolitis, stress, peroxide oxidation of lipids, antioxidant defense.Abstract
Goal. To study changes in the pro- and antioxidant system of the adrenal glands in guinea-pigs with experimental allergic alveolitis under stress conditions.
Materials and Methods. The studies were carried out on guinea pigs, in mature females weighing 0.18-0.20 kgs. The model of exogenous allergic alveolitis was reproduced by the method of O. O. Orhekhova, Yu.A. Kirilov The experimental model of acute "immobilisation stress" caused immobilization of animals at the operating table on the back, fixing atraumatically for the limbs. The duration of immobilization was 2 hours. The activity of lipid peroxidation was evaluated based on the content of diene conjugates and malonic dialdehyde at day 34 of the experiment. The functioning of the antioxidant system was assessed by the content of superoxide dismutase and catalase enzymes.
Results and Discussion. In the experimental group, we observed a probable increase in the values of malondialdehyde, diene conjugates, tapsuperoxide dismutase and catalase compared to the control group of animals. In animals with exogenous allergic alveolitis, the following changes occur under stress conditions: an increase in malondialdehyde, diene conjugates (p <0.05) and a probable increase in the activity of catalase and superoxide dismutase (p <0.05). Comparing the groups of animals with exogenous allergic alveolitis and the group of animals with exogenous allergic alveolitis under stress conditions, there was a decrease in the indices: diene conjugates, catalase activity (p <0,05).
Conclusions. The results of biochemical studies indicate that in the conditions of the development of experimental allergic alveolitis in animals, there are characteristic signs of the development of oxidative stress. This is manifested in the high intensity of oxidative reactions with excessive accumulation in the adrenal glands of products of free radical oxidation. When exposed to the organism of animals with experimental allergic alveolitis of stress, there is a decrease in the activation of the LPO processes and inhibition of antioxidant defense.
References
Khasky, A.D., & Smith, J.C. (2000). Stress, relaxation states, and creativity. Percept. Mot. Skills., 88 (22), 409-416.
Karanth, J., Jeevaratnam, K. (2005). Oxidative stress and antioxidant status in rat blood, muscle: effect of dietary lipid, carnitine and exercise. Bit. J. Vitam. Nutr. Res., 75, 5, 333-339.
Orekhov, A.A., Kirillov, Y.A. (1985). Patomorfologiya legkikh i mikrotsirkulyatornogo rusla malogo kruga krovoobrashcheniya pri khronicheskom eksperimentalnom allergicheskom alveolite [Patomorfology lung and microcirculatory of small circle in chronic experimentally allergic alveolitis]. Arkhiv patologii – Archive of Pathology, 10, 54-61 [in Russian].
Gavrilov, A., & Mishkorudnaya, M.I. (1989). Spektrofotometricheskoye opredeleniye soderzhaniya gidroperekisey lipidov v plazme. Laboratornaya diagnostika ishemicheskoy bolezni serdtsa [Spectrophotometric determination of lipid hydroperoxides in plasma. Laboratory diagnosis of coronary heart disease]. Kyiv: Zdorovye [in Russian].
Peddlers, E.N. (1989). Modifikatsiya opredeleniya produktov POL v reaktsii s tiobarbiturovoy kislotoy [Modification of the definition of lipid peroxidation products in the reaction with thiobarbituric acid]. Lab. Delo – Laboratory Case, 7, 8-10 [in Russian].
Fried, R. (1975). Enzymatic and nonenzymatic assay of super oxide dismutase Biochemie, 57 (5), 657-660.
Holmes, R., & Masters, C. (1970). Epigenetic interconversions of the multiple fonns of mouselivercatalase. FEBS Lett, 11 (l), 45-48.
Nykytiuk, H.P., & Porohovska, N.V. (2012). Korektsiia antyoksydantnoho zakhystu kardiomiotsytiv za umov hostroi eksperymentalnoi imunokompleksemii [Correction of antioxidant protection of cardiomyocytes with acute experimental immunocomplexemia]. Eksperymentalna ta klinichna fiziolohiia i biokhimiia – Experimental and Clinical Physiology and Biochemistry, 4, 35-39 [in Ukrainian].
Maruyama, W., Shaomoto-Nagai, M., Kato, Yo., Hisaka, Sh., Osawa, T., Naoi, M. (2014). Role of Lipid Peroxide in the Neurodegenerative Disorders Subcellular. Biochemistry, 77, 127-136.
Rogers, C., Davis, B., Dairell-Neufer, P., Murphy, M.P., Anderson, E.J., & Robidoux, J. (2014). A transient increase in lipid peroxidation primes preadipocytes for delayed mitochondrial inner membrane peraieabilization andATP depletion. Free Radical Biology and Medicine, 67, 330-341.
Khaichenko, V.V. (2007). Pryrodni biooksydanty ta pechinka [Natural biooksydants and liver]. Suchasna hastroenterolohiia – Modern Gastroenterology, 6 (38), 25-27 [in Ukrainian].
Perevozkina, M.G. (2013). Kataliticheskoye okisleniye mitsellyarnykh substratov v prisutstvii adrenalina i metildofa. Yestestvennyye i matematicheskiye nauki v sovremennom mire [Catalytic oxidation of micellar substrates in the presence of adrenaline and methyldopa. Natural and mathematical sciences in the modem world]. Materialy XII mezhdunarodnoy nauchno-prakticheskoy konferentsii – Materials of the XII International Scientific and Practical Conference, November 11 [in Russian].
