THE EFFECT OF IRON NANOPARTICLES ON OOCYTES AND SURROUNDING FOLLICULAR CELLS UNDER THE CONDITION OF IMMUNE COMPLEX-MEDIATED FAILURE
DOI:
https://doi.org/10.11603/1811-2471.2017.v0.i1.7483Keywords:
nanoparticulate zero-valent iron, experimental immune complex-mediated failure, oocyte meiotic maturation, apoptosis, necrosis.Abstract
The newest nanopharmacology way is to use of nanomedicines as substances for new medicines. Promising in this respect are nanoparticles of metals, particularly nanoparticulate zero-valent iron (nZVI). However, the possible protective mechanisms or the toxic effects of nZVI are not studied enough.
The aim of the work was to assess oocyte meiotic maturation, viability and DNA single-strand breaks of follicular cells surrounding oocytes (FCSO) under the conditions of experimental immune complex-mediated failure and treatment of iNOS inhibitor, donor NO and nZVI in mice. nZVI introduction leads to inhibition of MMO at Metaphase II and doesn`t cause significant alteration of cell viability parameters of FCSO, but there is some reallocation between nuclei of 0/1 and 2 classes upwards last compared with the corresponding values in the control. BSA immunization and treatment of L-arginine and nZVI lead to 1) improvement of MMO parameters at Metaphase I in relation to the corresponding values of BSA immunization and the introduction of L-arginine, but in relation to the corresponding values in the group under the conditions of BSA immunization and introduction of nZVI is likely inhibition; 2) reduction of cell death, namely, the number of living FCSO is increased, the number of cells with morphological signs of necrosis is reduced compared with the values in the BSA immunization and the introduction of L-arginine group. It has been shown that NO is involved in nZVI effects on oocytes and FCSO under the conditions of experimental immune complex-mediated failure.
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