CAG POLYMORPHISM RECEPTOR GENE ANDROGENS IN POSTMENOPAUSAL WOMEN WITH HYPERTENSION AND DIABETES
DOI:
https://doi.org/10.11603/1811-2471.2016.v0.i4.7070Keywords:
hypertension, diabetes, polymorphism gene AR, DHT, menopause.Abstract
The aim of the work was to investigate the relationship between repetitions CAG- repeats AR gene and levels of dihydrotestosterone (DHT) in the serum of postmenopausal women (PM), with diabetes mellitus (DM) type 2 and hypertension (HT).
106 women in PM were divided into groups: the first included 30 patients with type 2 diabetes; the second – 34 patients with hypertension 2–3 grade; the 3rd – 42 patients with hypertension of –3 degree and type 2 diabetes, the control group – 31 women. All women ELISA method DHT levels measured in serum isolated genomic DNA from white blood cells, were identified number of repetitions CAG- repeats AR gene PCR.
CAG-repeats were observed in 96 (70.07 ± 3.91 %) of 137 cases. The minimum number of CAG-repeats equal to 14 maximum - 28 AR gene allele prevalence was significantly different depending on the groups of patients. To determine the influence of gene polymorphisms AR at risk of developing the disease was a comparative analysis of long alleles between groups monitoring via indicator SBM. For critical number of CAG-repeats were taken 22 repetitions. Among indicators of DHT in serum and CAG-repeats in the AR gene observed groups of women observed a direct correlation.
AG as concomitant disease, adversely affect the course of type 2 diabetes in women PM, resulting in greater levels of DHT increase in venous blood in patients with comorbid disorders than in patients with type 2 diabetes.
The relation between the level of DHT in the blood plasma and the number of CAG-repeats in PM women, a strong correlation was between DHT and "long" alleles AR gene in the group with comorbid disorders (hypertension with type 2 diabetes). The presence of increased number of CAG-repeats in AR gene can be regarded as a predictor of the development and progression of hypertension in women with type 2 diabetes in PM.
References
Elektronnyy resurs. World Health Organization Statistical Information System. 2009. Rezhym dostupu: www.who.int/whosis.
The DECODE study group on behalf of the European Diabetes Epidemiology Grour. 1999. Glucose tolerance and mortality: comparisonof WHO and American Diabetic Association diagnostic criteria. The DECODE study group on behalf of the European Diabetes Epidemiology Grour. Lancet. –1999. – Vol. 354 (9179). – R. 617–621.
Ed. by Armen Yuri Gasparya. 2012. Cardiovascular Risk Factors / Gender-Specific Aspects in the Clinical Presentation of Cardiovascular Disease. InTech: Chapter 14. – 2012. – 504r.
Butov O. D. Association of interleukins genes polymorphisms with multi-drug resistant tuberculosis in Ukrainian population / O. D. Butov, M. M. Kuzhko, S. T. Butova // Pneumonologia i Alergologia Polska. – 2016. – Vol. 84, No. 3. – P. 168–173.
Androgen Therapy in Women: An Endocrine Society Clinical Practice / M. E. Wierman, R. Basson, S. R. Davis, M. E. Wierman // J. Clin. Endocrinol. Metabol. – 2006. – Vol. 91 (10). – P. 3697–3716.
Kalynchenko S.YU. & Apetov S.S. 2010. Rolʹ androhenov u zhenshchyn: chto my znaem? Lech vrach. – 2010(№8). – S.78–83.
Gao W. Chemistry and structural biology of androgen receptor / W. Gao, C. E. Bohl, Y. T. Dalton // Chem. Rev. 2005. – Vol. 105(9). – R. 3352–3370.
Gobinet J. Molecular action of androgens / J. Gobinet, N. Pujol, C. Sultan // Mol. Cell. Endocrinol. – 2002. – Vol. 198. – R. 15–24.
Reduced androgen receptor gene expression with first exon CAG repeat expansion / C. S. Choong, J. A. Kemppainen, Z. X. Zhou, E. M. Wilson // Mol. Endocrinol. – 1996. – Vol. 10, No. 12. – P. 1527–1535.
Effect of a short CAG (glutamine) repeat on human androgen receptor function / D. Ding, L. Xu, M. Menon [et al.] // Prostate. – 2004. – Vol. 58(1). – P. 23–32.
Formation of the androgen receptor transcription complex / Y. Shang, M. Myers, M. Brown // Mol.Sell. – 2002. – Vol. 9(3). – P. 601–610.
Gelmann E. P. Molecular biology of the androgen receptor / E. P. Gelmann // J. Clin. Oncol. – 2002. – Vol. 20(13). – P. 3001–3015.
Tsigos C. Management of obesity in adults: European clinical practice guidelines / C. Tsigos, V. Hainer, A. Basdevant // Obesity Facts. – 2008. – Vol. 1, No. 2. – P. 106–116.
Zhuravleva L. V. Sovremennaya stratehyya vedenyya patsyentov s arteryalʹnoy hypertenzyey / L. V. Zhuravleva, N. A. Lopyna // Zdorovʺya Ukrayiny. – 2014. – № 1 (32). – S. 22–24.
Svishchenko YE. P. Rekomendatsiyi Ukrayinsʹkoyi asotsiatsiyi kardiolohiv z profilaktyky ta likuvannya arterialʹnoyi hipertenziyi : posibnyk do Natsionalʹnoyi prohramy profilaktyky i likuvannya arterialʹnoyi hipertenziyi (4-e vyd.) / Svishchenko YE. P., Bahriy A. E. – K. : NNTS Instytut kardiolohiyi Ukrayiny im. M. D. Strazheska, 2012. – 53 s.
Lapach S. N. Statystycheskye metody v medykobyolohycheskykh yssledovanyyakh s yspolʹzovanyem Excel / S. N. Lapach, A. V. Chubenko, P. N. Babych – K. : Moryon, 2000. – 320 s.
