CHANGES IN THE ACTIVITY OF ENERGY PROCESSES IN THE ORGANISM OF RATS WITH EXPERIMENTAL ADRENALINE CARDIOPATHIES
DOI:
https://doi.org/10.11603/1811-2471.2024.v.i4.15056Keywords:
adrenaline, white rats, heart, liver, succinate dehydrogenase activity, cytochrome oxidase activity, glucose, glycogen, energy processesAbstract
SUMMARY. In the conditions of socio-economic instability, moral and mental stress that Ukraine has been experiencing over the past decade, the influence of stress factors on the occurrence of cardiac pathology has become significantly more widespread. With pathologically enhanced adrenergic influence, tissue respiration processes are suppressed, which leads to the development of hypoxia in this pathology.
The aim – to investigate the activity of mitochondrial enzymes and the main energy substrates of the myocardium in the body of rats with heart damage caused by high doses of adrenaline.
Material and methods. The studies were conducted on white male rats weighing 170-200 g, which were kept on a standard diet of the vivarium of the National Medical University of Medical Sciences. The rats were divided into two groups: the 1st intact control group, the 2nd experimental group, in which myocardial damage was caused by a single intramuscular injection of 0.18 % adrenaline hydrotartrate solution at a dose of 0.5 mg/kg body weight. Euthanasia was performed using sodium thiopental at the 3rd, 24th and 48th hour after the injection of adrenaline in compliance with all the rules for working with vertebrate animals. The heart, liver and blood serum of the animals were subjected to the study. Succinate dehydrogenase and cytochrome oxidase activity, as well as glycogen content, were determined in the heart and liver, and glucose content in the blood serum. Parametric (Student's t-test) and nonparametric (Wilcoxon's) methods were used for statistical analysis. Changes were considered significant at p≤0.05.
Results. Exposure of rats to high doses of adrenaline caused a progressive decrease in succinate dehydrogenase activity within 48 hours in the heart of rats. At the end of the study, this indicator decreased by 1.45 times. In the liver of animals exposed to adrenaline, succinate dehydrogenase activity progressively decreased at all times and was maximally lower than the control by 22 % within 3 hours after exposure. Cytochrome oxidase activity under conditions of exposure to high doses of adrenaline was sharply suppressed in the myocardium compared to the intact group of rats. In 48 hours after the administration of adrenaline in the myocardium, it decreased by 1.5 times. The lowest level of cytochrome oxidase activity in the liver was recorded 48 hours after the administration of high doses of adrenaline. Under the influence of adrenaline, there was an increase in the blood serum of rats at all times of the study, with maximum values 3 hours after the lesion. Within 48 hours after the lesion with adrenaline, a reduced glycogen content was noted in the heart of rats. The lowest indicator was recorded 3 hours after the start of the experiment. A similar decrease in glycogen content was noted in the liver throughout the study, with the most pronounced decrease in this indicator observed at 3 hours.
Conclusions. The results of our studies confirm profound disturbances in energy metabolism in the body of rats under conditions of adrenaline myocardial damage, which is manifested by a decrease in the activity of the main bioenergetic enzymes and negative dynamics of the content of energy substrates.
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