PROTECTIVE EFFECT OF WATER ENRICHED WITH MOLECULAR HYDROGEN IN EXPERIMENTAL COLORECTAL CANCER
DOI:
https://doi.org/10.11603/1811-2471.2024.v.i4.15052Keywords:
molecular hydrogen, oxidative stress, cGMP, colorectal cancerAbstract
SUMMARY. Oxidative processes play an important role in the pathogenesis of oncogenesis, e. g. colorectal cancer (CRC). There are also a number of reports on changes in the functioning of the cyclic nucleotide system in cancer patients, in particular, on the role of cGMP. The use of antioxidants can potentially slow down the tumor process. Given the antioxidant and anti-inflammatory properties of molecular hydrogen, it is relevant to study the potential therapeutic effect of hydrogen-enriched water in this oncopathology.
The aim – to assess the effect of water saturated with molecular hydrogen on the content of cGMP and malondialdehyde (MDA) in the blood serum of rats with CRC treated with 5-fluorouracil.
Material and Methods. Experiments were conducted on 90 male Wistar white rats. CRC was simulated in animals by subcutaneous injection of 1,2-dimethylhydrazine (1,2-DMG) at a dose of 7.2 mg/kg once a week for 30 weeks. Rats consumed water saturated with molecular hydrogen at a concentration of 0.6 ppm ad libitum. In blood serum the content of cGMP was measured by immunoassay method and the level of MDA was measured by the colorimetric method.
Results. The content of cGMP in the blood serum of rats with CRC was 39.9 % lower than in animals of the control group, while the level of MDA in the blood serum increased by 1.9 times. The content of cGMP in the blood serum of rats with CRC that consumed water enriched with molecular hydrogen was 38.1 % higher than in animals that consumed tap water, while the level of MDA significantly (by 30.8 %) decreased. Administration of 5-FU to animals with CRC led to a decrease in the content of cGMP in the blood serum by 42.3 % and an increase in MDA by 2.3 times compared to the control, but did not significantly differ from similar indicators in animals with CRC that were not treated with 5-FU. Serum cGMP levels in animals treated by 5-FU consuming hydrogen-enriched water were 24.1 % higher than in 5-FU-treated CRC rats that had access to tap water. Hydrogen water did not significantly affect the level of oxidative stress in 5-FU-treated CRC rats.
Conclusions. In rats with CRC induced by 1,2-DMG, the level of blood serum cGMP decreased and the content of MDA increased. Treatment of CRC-rats with 5-FU does not lead to significant changes in the intensity of oxidative stress and the cyclic nucleotide system. Consumption of water saturated with molecular hydrogen by animals with CRC leads to a significant improvement in the intensity of oxidative stress and the cyclic nucleotide system.
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