QUANTITATIVE MORPHOLOGICAL ASSESSMENT OF THE AGE REMODELING OF THE VENOUS BED OF THE LEFT AND RIGHT ATRIA IN EXPERIMENTAL ANIMALS
DOI:
https://doi.org/10.11603/1811-2471.2024.v.i3.14719Keywords:
left, right atrium, venous vessels, age-related changes, morphometryAbstract
SUMMARY. Cardiovascular diseases are an important medical and social problem today. Recently, significant progress has been made in the diagnosis, correction and prevention of heart lesions, but the structural and functional features of the myocardium continue to be studied by morphologists and clinicians.
The aim – to investigate the age-related patterns of quantitative morphological changes in the venous vessels of the atria in laboratory sexually mature white male rats.
Material and methods. The complex of morphological methods studied the venous bed of the left and right atrium of 40 male rats, which were divided into 2 groups. The 1-st group included 20 intact animals aged 6 months, the 2-nd – 20 rats aged 24 months. Animals were euthanized by bloodletting under thiopental anesthesia. Histological preparations were made from the atria, on which the diameters of postcapillary venules, venules, outer and inner diameters, wall thickness of venous vessels, area of endotheliocytes, their nuclei, nuclear-cytoplasmic ratio in these cells, relative volumes of damaged endotheliocytes were determined. Morphometric parameters were processed statistically.
Results. Remodeling of the venous vessels of the atria occurs with age. Thus, the diameter of postcapillary venules increased by 6.2 %, venules by 5.7 %. In the right atrium of 24-month-old animals, the specified morphometric parameters changed by 5.7 % and 5.1 %, respectively (р<0.001). The thickness of the wall of the venous vessels of the left atrium decreased by 3.2 % with age, the relative volume of damaged endotheliocytes increased by 2.3 times, in the right atrium by 3.3 % and 2.1 times, respectively.
Conclusions. The conducted studies and the obtained results show that with age, pronounced remodeling of the veins of the atria occurs in laboratory sexually mature white male rats, which is characterized by the expansion of the venous link of the hemomicrocirculatory bed, venous vessels, a decrease in the thickness of their walls, atrophy of endotheliocytes, an increase in the relative volume of damaged endotheliocytes at preserved stability of cellular structural homeostasis. The degree of age-related structural reorganization of the venous bed dominates in the postcapillary venules and venules of the left atrium of 24-month-old experimental animals.
References
Aksonov, E.V. (2019). Endotelial'na dysfunktsiya ta shlyakhy yiyi profilaktyky pry provedenni renthenendovaskulyarnykh protsedur po rekanalizatsiyi koronarnykh arteriy [Endothelial dysfunction and ways of its prevention during X-ray endovascular procedures for recanalization of coronary arteries]. Ukrayins'kyy zhurnal medytsyny, biolohiyi ta sportu – Ukrainian Journal of Medicine, Biology and Sports, 5(21),105-108 [in Ukrainian]. DOI: 10.26.693/jmbs04.05.109.
Bahrii, M.M., Dibrova, V.A., Popadynets, O.H., & Hryshchuk, M.I. (2016). Metodyky morfolohichnykh doslidzhen [Methods of morphological research]. Vinnytsia: Nova knyha [in Ukrainian].
Baibakov, V.M. (2011). Morfofunktsional'ni zminy venoznoho rusla yak lanky drenazhnoyi systemy yayechka pry travmuvanni sudynnykh anastomoziv sim'yanoho kanatyka v eksperymenti [Morphofunctional changes of the venous bed as part drainage system of the testis in trauma to the vascular anastomoses of the spermatic cord of the experiment]. Klinichna anatomiya i operatyvha khirurhiya – Clinical Anatomy and Operative Surgery, 4(16), 32-35 [in Ukrainian].
Zaporozhyan, V.M., & Aryaev, M.L. (2013). Bioetyka i biobezpeka [Bioethics and biosafety]. Kyiv: Zdorovia [in Ukrainian].
Kuzyk, Yu.I. (2019). Features of the pathomorphological structure of the aterosclerotic plaques of carotid atherosclerosis. Pathologia, 3, 355-361. DOI: 10.14739/ 2310-1237.2019.3.188872
Malyarska, N.V., & Kalinichenko, M.A. (2017). Endotelial'na dysfunktsiya yak universal'nyy predyktor rozvytku sertsevo-sudynnoyi patolohiyi ta mozhlyvosti yiyi korektsiyi v praktytsi simeynykh likariv [Endothelial dysfunction as a universal predictor of cardiovascular pathology and the possibility of its correction in the practice of family physicians]. Liky Ukraiyiny – Medicines of Ukraine, 1(207), 38-41 [in Ukrainian].
Nesteruk, S.O., Tatarchuk, L.V., Hnatyuk, M.S., & Monastyrska, N.Y a. (2023). Kil'kisne morfolohichne vyvchennya osoblyvostey porushen' venoznoho drenazhu sim’yanykiv v umovakh etanolovoyi intoksykatsiyi [Quantitative morphological study of the peculiarities of violations of the venous drainage of testicles in conditions of ethanol intoxication]. Zdobutky klinichnoyi ta eksperymental'noyi medytsyny – Achievements of clinical and experimental medicine, 3, 135-139 [in Ukrainian].
Khlamanova, L.I., Severilova, M.D., & Tkachenko, Ju.V. (2021). Morfofunktsional'ni osoblyvosti apoptozu. Problemy ta perspektyvy zastosuvannya apoptozu v suchasniy medytsyni [Morphofunctional features of apoptosis. Problems and prospects of apoptosis in modern medicine]. Ukrayins'kyy zhurnal medytsyny i sportu – Ukrainian Journal of Medicine, Biology and Sports, 2(4), 185-191 [in Ukranian].
Petrie, A., & Sabin, C. (2019). Medical statisties at a Glance.4th ed. New York: Wiley.
