QUANTITATIVE MORPHOLOGICAL ANALYSIS OF AGE CHANGES OF SPATIAL CHARACTERISTICS OF HEART CHAMBERS IN CONDITIONS OF COBALT CHLORIDE INTOXICATION
DOI:
https://doi.org/10.11603/1811-2471.2024.v.i2.14684Keywords:
cobalt chloride, parts of the heart, age changes, planimetric, volumetric parametersAbstract
SUMMARY. Cardiovascular pathology is an important medical and social problem today. Despite the successes in the diagnosis, prevention and correction of heart lesions, the structure and function of which continue to be studied by morphologists and clinicians.
The aim – to study the features of age-related changes in planimetric and volumetric parameters of parts of the heart of experimental animals during intoxication with cobalt chloride using quantitative morphological methods.
Material and Methods. Planimetric and volumetric measurements of the heart parts of 120 laboratory sexually mature white male rats, which were divided into 4 groups of 30 animals each, were carried out. The first and second groups were control. The third group (six-month-old rats) and the fourth group (24-month-old rats) animals were daily intragastrically injected with cobalt chloride at a dose of 4 mg/kg for 30 days. Animals were euthanized by bloodletting under thiopental anesthesia. Following euthanasia, the planimetry of the chambers and determination of the supply, output and reserve volumes of the ventricles of the heart was carried out. Quantitative indicators were statistically analyzed.
Results. Long-term intoxication of experimental animals with cobalt chloride leads to uneven, disproportionate expansion of the chambers of the heart, which was confirmed by pronounced changes in the planimetric index and planimetric index of the atria. At the same time, a pronounced increase in the volume of the inflow and outflow chambers of the ventricles of the heart and a decrease in their reserve volumes were established. The latter in the left and right ventricles of the six-month-old rats decreased by 50.8 % and 21.0 % (p<0.001), respectively, while in the 24-month-old rats – by 51.3 % and 41.5 %, respectively (p<0.001).
Conclusions. The conducted studies and the obtained results indicate that intoxication of laboratory white male rats with cobalt chloride leads to uneven, disproportionate expansion of the heart chambers, which confirm changes in the areas of the endocardial surfaces of the ventricles and atria, the planimetric index and the planimetric index of the atria, the increase in supply and output, and a decrease in reserve volume of the ventricles. The most pronounced age-related decrease in the reserve volume was found in the left ventricle.
References
Hnatjuk, M.S., Gdanska, N.M., Tatarchuk, L.V., & Monastyrska, N.Ya. (2021). Morfometrychni aspekty vyvchennya prostorovykh zmin kamer sertsya pry rezektsiyi riznykh ob˝yemiv parenkhimy pechinky [Morphometric aspects of the study of spatial changes in heart chambers during resection of different volumes of liver parenchyma]. Visnyk problem biolohiyi i medytsyny – Herald of problems of biology and medicine, 3 (161), 249-252 [in Ukrainian].
Mishalov, V.D., Tverdokhlib, I.V, & Yurchenko, V.T. (2016). Pravove ta zakonodavche obgruntuvannya poryadku vyluchennya biolohichnykh ob˝yektiv vid trupa lyudyny dlya naukovykh doslidzhen´ z anatomiyi, histolohiyi, tsytolohiyi [Legal and legislative study of biological, objects be withdrawn from human courpse for scientific research in anatomy, histology, cytology]. Morphologia – Morphologia, 1 (10), 107-110 [in Ukranian].
Zaporozhyan, V.M., & Aryaev, M.L. (2013). Bioetyka i biobezpeka [Bioethics and biosafety]. Kyiv: Zdorovia [in Ukrainian].
Hnatiuk, M.S., Slabyi, O.B., & Hasiuk, P.A. (2016). Osoblyvosti remodeliuvannia kamer sertsia zalezhno vid typiv tsentralnoi hemodynamiky [Features of heart chamber remodeling depending on the types of central hemodynamics]. Svit medytsyny i biolohii – World of Medicine and Biology, 4 (58), 124-127 [in Ukrainian].
Akin, I., & Nienaber, С. (2020). Prediction of aortic dissection. Heart, 106 (12), 870-871. https://doi.org/ 10.1136/heartjnl-2020-316617
Amzulescu, M., Slavich, M., Florian, A., & Goetschalckx, K. (2013). Does two-dimensional image reconstruction from three-dimensional full volume echocardiography improve the assessment of left ventricular morphology and function? Echocardiography, 30(1), 55-63. DOI: 10.1111/j.1540-8175.2012.01800.x
Festing, M., & Nevalainen, T. (2014). The design and statistical analysis of animal experiments introduction to this issue. Ilar Journal, 55(3), 379 –372.
Kuzyk, Yu.I. (2019). Features of the pathomorphological structure of the aterosclerotic plaques of carotid atherosclerosis. Pathologia, 3, 355-361. https://doi.org/ 10.14739/2310-1237.2019.3.188872
Sataieyva, T.P., Zadnipryany, I.V., & Zukov, W. (2019). Toxic cardiac effect of cobalt chloride excessive chronic consumption in male Wistar rats. Ecological question, 3, 25-32. DOI: http://dx.dji.org/1012775/EQ.2019.016
Vlasiuk, T.I., & Zhurakivska, O.Y. (2018). Prospects for the use of exenatide in the correction of experimental diabetic cardiomyopathies. The Pharma Innovation Journal, 7(9), 35–40.
