RELATIONSHIP BETWEEN INFECTION WITH HUMAN PAPILLOMA VIRUS AND PROBLEMS WITH FERTILITY OF WOMEN AND MEN IN MARRIED COUPLES
DOI:
https://doi.org/10.11603/1811-2471.2024.v.i1.14533Keywords:
human papilloma virus, spermatogenesis, married couplesAbstract
SUMMARY. The aim – detection of the possible impact of HPV on reproductive capacity.
Material and Methods. The study was conducted on 78 couples who underwent IVF. An analysis of clinical records, laboratory studies and the results of the use of assisted reproduction methods was carried out in the form of a retrospective study. Men were subjected to sperm analysis. The nucleic acid amplification method was used to detect HPV types. QIAamp DNA Blood Mini Kit from QIAGEN was used for DNA isolation.
Results. The analysis showed that HPV infection was common among 52.5 % of couples (41/78), affecting 38.5 % of women and 46.1 % of men. Multiple HPV infections were detected in 7 semen samples. In 14 couples (15.6 %), HPV infection was detected in both partners. HPV type 58 was most common in women (46.6 %), HPV type 52 in semen (44.4 %), and other HPV types were also found in numerous men and women.
In the study, 50 % of the samples had normospermia, and the other 50 % showed abnormalities in sperm quality, including oligospermia and asthenospermia. HPV infections were less common in normospermic sperm samples (10 %) compared to abnormal samples (36.6 %). Comparing sperm parameters in patients with and without HPV, a statistically significant difference in sperm quality was found. Men with abnormalities have more HPV-positive samples (68.3 %), compared to HPV-negative (55.5 %).
Conclusions. HPV can affect spermatogenesis, particularly sperm quality. HPV 52 was the most common among couples treated for infertility, especially in the case of low concentration and reduced sperm motility. This indicates a possible effect of HPV 52 on male infertility.
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