RELATIONSHIP BETWEEN INFECTION WITH HUMAN PAPILLOMA VIRUS AND PROBLEMS WITH FERTILITY OF WOMEN AND MEN IN MARRIED COUPLES
DOI:
https://doi.org/10.11603/1811-2471.2024.v.i1.14533Keywords:
human papilloma virus, spermatogenesis, married couplesAbstract
SUMMARY. The aim – detection of the possible impact of HPV on reproductive capacity.
Material and Methods. The study was conducted on 78 couples who underwent IVF. An analysis of clinical records, laboratory studies and the results of the use of assisted reproduction methods was carried out in the form of a retrospective study. Men were subjected to sperm analysis. The nucleic acid amplification method was used to detect HPV types. QIAamp DNA Blood Mini Kit from QIAGEN was used for DNA isolation.
Results. The analysis showed that HPV infection was common among 52.5 % of couples (41/78), affecting 38.5 % of women and 46.1 % of men. Multiple HPV infections were detected in 7 semen samples. In 14 couples (15.6 %), HPV infection was detected in both partners. HPV type 58 was most common in women (46.6 %), HPV type 52 in semen (44.4 %), and other HPV types were also found in numerous men and women.
In the study, 50 % of the samples had normospermia, and the other 50 % showed abnormalities in sperm quality, including oligospermia and asthenospermia. HPV infections were less common in normospermic sperm samples (10 %) compared to abnormal samples (36.6 %). Comparing sperm parameters in patients with and without HPV, a statistically significant difference in sperm quality was found. Men with abnormalities have more HPV-positive samples (68.3 %), compared to HPV-negative (55.5 %).
Conclusions. HPV can affect spermatogenesis, particularly sperm quality. HPV 52 was the most common among couples treated for infertility, especially in the case of low concentration and reduced sperm motility. This indicates a possible effect of HPV 52 on male infertility.
References
Huang, C., Wang, Y., Li, X., Ren, L., Zhao, J., Hu, Y., ... & Cao, B. (2020). Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China. The lancet, 395(10223), 497-506. DOI: https://doi.org/10.1016/S0140-6736(20)30183-5
Guan, W-J, Zheng-yi, N, Yu, H, Liang, W-H, Chun-quan, O, Jian-xing, H, Liu, L, … Lan-juan, L (2020). Clinical characteristics of coronavirus disease 2019 in China. New England Journal of Medicine, 382, 1708-1720. DOI: https://doi.org/10.1056/NEJMoa2002032
Chen, N., Zhou, M., Dong, X., Qu, J., Gong, F., Han, Y., ... & Zhang, L. (2020). Epidemiological and clinical characteristics of 99 cases of 2019 novel coronavirus pneumonia in Wuhan, China: a descriptive study. The lancet, 395(10223), 507-513. DOI: https://doi.org/10.1016/S0140-6736(20)30211-7
Chen, T., Wu, D. I., Chen, H., Yan, W., Yang, D., Chen, G., ... & Ning, Q. (2020). Clinical characteristics of 113 deceased patients with coronavirus disease 2019: retrospective study. bmj, 368. DOI: https://doi.org/10.1136/bmj.m1091
Perino, A., Giovannelli, L., Schillaci, R., Ruvolo, G., Fiorentino, F.P., Alimondi, P., ... & Ammatuna, P. (2011). Human papillomavirus infection in couples undergoing in vitro fertilization procedures: impact on reproductive outcomes. Fertility and sterility, 95(5), 1845-1848. DOI: https://doi.org/10.1016/j.fertnstert.2010.11.047
Zacharis, K., Messini, C.I., Anifandis, G., Koukoulis, G., Satra, M., & Daponte, A. (2018). Human papilloma virus (HPV) and fertilization: a mini review. Medicina, 54(4), 50. DOI: https://doi.org/10.3390/medicina54040050
Çağlar, G.S., & Garrido, N. (2018). The implications of male human papilloma virus infection in couples seeking assisted reproduction technologies. Journal of the Turkish German Gynecological Association, 19(1), 48. DOI: https://doi.org/10.4274/jtgga.2017.0031
Hellberg, D., & Nilsson, S. (2007). IVF and HPV. Fertility and Sterility, 87(6), 1498. DOI: https://doi.org/10.1016/j.fertnstert.2007.01.135
Souho, T., Benlemlih, M., & Bennani, B. (2015). Human papillomavirus infection and fertility alteration: a systematic review. PloS One, 10(5), e0126936. DOI: https://doi.org/10.1371/journal.pone.0126936
Bruni, L., Albero, G., Rowley, J., Alemany, L., Arbyn, M., Giuliano, A.R., ... & Taylor, M. (2023). Global and regional estimates of genital human papillomavirus prevalence among men: a systematic review and meta-analysis. The Lancet Global Health, 11(9), e1345-e1362. DOI: https://doi.org/10.1016/S2214-109X(23)00305-4
Chesson, H.W., Dunne, E.F., Hariri, S., & Markowitz, L.E. (2014). The estimated lifetime probability of acquiring human papillomavirus in the United States. Sexually Transmitted Diseases, 41(6), 660-664. DOI: 10.1097/OLQ.0000000000000169. DOI: https://doi.org/10.1097/OLQ.0000000000000193
Giuliano, A.R., Anic, G., & Nyitray, A.G. (2010). Epidemiology and pathology of HPV disease in males. Gynecologic Oncology, 117, S15-S19. DOI: https://doi.org/10.1016/j.ygyno.2010.01.026
Hermonat, P.L., Han, L., Wendel, P.J., Quirk, J.G., Stern, S., Lowery, C.L., & Rechtin, T.M. (1997). Human papillomavirus is more prevalent in first trimester spontaneously aborted products of conception compared to elective specimens. Virus Genes, 14, 13-17. DOI: https://doi.org/10.1023/A:1007975005433
Moghimi, M., Zabihi-Mahmoodabadi, S., Kheirkhah-Vakilabad, A., & Kargar, Z. (2019). Significant correlation between high-risk HPV DNA in semen and impairment of sperm quality in infertile men. International Journal of Fertility and Sterility, 12, 306-309.
Elbashir, S., Magdi, Y., Rashed, A., Ibrahim, M.A., Edris, Y., & Mostafa Abdelaziz, A. (2018). Relationship between sperm progressive motility and DNA integrity in fertile and infertile men. Middle East Fertility Society Journal, 23, 195-198. DOI: https://doi.org/10.1016/j.mefs.2017.12.002
Nasseri, S., Monavari, S.H., Keyvani, H., Nikkhoo, B., Vahabpour Roudsari, R., & Khazeni, M. (2015). The prevalence of Human Papilloma Virus (HPV) infection in oligospermic and azoospermic men. Medical Journal of the Islamic Republic of Iran, 29, 272-272.
Garolla, A., Pizzol, D., & Foresta, C. (2011). The role of human papillomavirus on sperm function. Current Opinion in Obstetrics and Gynecology, 23(4), 232-237. DOI: 10.1097/GCO.0b013e328348a3a4. PMID: 21666465. DOI: https://doi.org/10.1097/GCO.0b013e328348a3a4
Yang, Y., Jia, C.W., Ma, Y.M., Zhou, L.Y., & Wang, S.Y. (2013). Correlation between HPV sperm infection and male infertility. Asian J. Androl., 15(4), 529-532. DOI: https://doi.org/10.1038/aja.2013.36
Gizzo, S., Ferrari, B., Noventa, M., Ferrari, E., Patrelli, T.S., Gangemi, M., ... & Nardelli, G.B. (2014). Male and couple fertility impairment due to HPV-DNA sperm infection: Update on molecular mechanism and clinical impact-Systematic review. Biomed. Research International, 230-263. DOI: https://doi.org/10.1155/2014/230263
Rintala, M.A., Grenman, S.E., Pollanen, P.P., Suominen, J.J., & Syrjanen, S.M. (2004). Detection of high-risk HPV DNA in semen and its association with the quality of semen. International Journal of STD & AIDS, 15(11), 740-743. DOI: https://doi.org/10.1258/0956462042395122
