APPLICATION OF THE INDIRECT IMMUNOFLUORESCENCE METHOD (BIOCHIP TECHNOLOGY) FOR THE DIAGNOSIS OF EPSTEIN-BARR VIRUS INFECTION IN RESIDENTS OF TERNOPIL REGION
DOI:
https://doi.org/10.11603/1811-2471.2023.v.i4.14312Keywords:
hypothyroidism, metabolic syndrome, insulin resistance, treatmentAbstract
SUMMARY. The aim – to diagnose EBV infection in residents of Ternopil region by the content of serum antibodies of classes M and G to the EBV antigens: capsid and its proteins gp125 and p19, antibodies of class G – to early and nuclear antigens, using the multiplex indirect immunofluorescence method in the BIOCIP technology.
Material and Мethods. At the Сentre for Lyme borreliosis and other tick-borne diseases research at the I. Horbachevsky Ternopil National Medical University of the Ministry of Health of Ukraine, 26 patients aged 30 to 72 years with clinical manifestations of EBV infection were examined. There were 7 men (26.9 %), and the majority of the patients were women – 19 (73.1 %). 18 (69.2 %) people lived in the city, 8 (30.8 %) in the countryside.
For the diagnosis of EBV infection, the multiplex indirect immunofluorescence method in the BIOCIP technology was performed, using the commercial kit (EUROIMMUN BIOCHIP Sequence EBV Avidity Determinant; Lot: FI 2799-21 X, Germany), which contains capsid antigen and its proteins gp125 and p19, nuclear and early EBV antigens. The results of determination of specific antibodies to these EBV antigens were evaluated with a fluorescence microscope (Olympus IX70; ok ×10; ob × 20, 40 objective) by the bright green glow of the fluorescein-labeled antigen-antibody immune complex, which was specific for each of these antigens.
Results. The multiplex indirect immunofluorescence method in the BIOCIP technology allowed to diagnose chronic EBV infection in all examined patients, and due to the simultaneous detection of different combinations of IgG to the capsid antigen of EBV and its proteins gp125 and p19 as well as to the nuclear and early antigens, we were able to establish the stage of reactivation of EBV infection in 46.2 % of patients, long-standing infection in 34.6 %, and chronic infection with recent reactivation in 19.2 %.
In patients with chronic EBV infection, complaints of swollen lymph nodes (56 %) and muscle and joint pain (36 %) were more common.
Conclusion. This method for the diagnosis of chronic EBV infection in the Ternopil region was used for the first time and demonstrated high information content.
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